Pathology of callosal damage in ALS: An ex-vivo , 7 T diffusion tensor MRI study

NeuroImage. Clinical
Agustin M CardenasMary Kay Floeter

Abstract

The goal of this study was to better understand the changes in tissue microstructure that underlie white matter diffusion changes in ALS patients. Diffusion tensor imaging was carried out in postmortem brains of 4 ALS patients and two subjects without neurological disease on a 7 T MRI scanner using steady-state free precession sequences. Fractional anisotropy (FA) was measured in the genu, body, and splenium of the corpus callosum in formalin-fixed hemispheres. FA of the body and genu was expressed as ratio to FA of the splenium, a region unaffected in ALS. After imaging, tissue sections of the same segments of the callosum were stained for markers of different tissue components. Coded image fields were rated for pathological changes by blinded raters. The FA body/FA splenium ratio was reduced in ALS patients compared to controls. Patchy areas of myelin pallor and cells immunostained for CD68, a microglial-macrophage marker, were only observed in the body of the callosum of ALS patients. Blinded ratings showed increased CD68 + microglial cells in the body of the corpus callosum in ALS patients, especially those with C9orf72 mutations, and increased reactive astrocytes throughout the callosum. Reduced FA of the corpus callosum i...Continue Reading

Citations

Apr 19, 2018·Journal of Neurology, Neurosurgery, and Psychiatry·Robert D HendersonAndrew Eisen
Jul 12, 2019·Amyotrophic Lateral Sclerosis & Frontotemporal Degeneration·Peter Bede
Feb 15, 2020·Journal of Neurology, Neurosurgery, and Psychiatry·Michael SwashMatthew C Kiernan
Feb 29, 2020·Amyotrophic Lateral Sclerosis & Frontotemporal Degeneration·Sicong TuMartin R Turner
Apr 16, 2020·Amyotrophic Lateral Sclerosis & Frontotemporal Degeneration·Peter Bede, Rangariroyashe H Chipika
Dec 8, 2017·Amyotrophic Lateral Sclerosis & Frontotemporal Degeneration·Peter Bede, Orla Hardiman
Feb 6, 2020·Current Medical Imaging Reviews·Rodolfo Gabriel Gatto, Carina Weissmann
Jun 20, 2020·Nature Reviews. Neuroscience·Peter McColganGeraint Rees
Apr 4, 2019·Frontiers in Neurology·Rangariroyashe Hannah ChipikaPeter Bede
Apr 10, 2018·Expert Review of Neurotherapeutics·Federica AgostaMassimo Filippi
Apr 6, 2021·Therapeutic Advances in Chronic Disease·Hans-Peter MüllerJan Kassubek
Oct 12, 2021·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·Aya OguraGen Sobue

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Methods Mentioned

BETA
transgenic

Software Mentioned

FMRIB
DTIFit
Medical Image Processing , Analysis and Visualization
TORTOISE
DTI Studio

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