Pathophysiology of antiphospholipid antibodies: absence of prostaglandin-mediated effects on cultured endothelium

American Journal of Obstetrics and Gynecology
D J DudleyD W Branch

Abstract

Antiphospholipid antibodies are associated with recurrent pregnancy loss and thrombosis. Studies suggest that antiphospholipid antibodies may inhibit the production of prostacyclin by vascular tissues. We incubated sera from women with moderate to high levels of antiphospholipid antibodies with primary human umbilical vein endothelial cell cultures. Intact confluent, subconfluent, hydrogen peroxide-damaged, and mechanically damaged endothelial cell monolayers were evaluated for the production of the prostacyclin metabolite 6-keto-prostaglandin F1 alpha in the presence of sera that were positive and negative for antiphospholipid antibodies. All sera were assayed for baseline concentrations of 6-keto-prostaglandin F1 alpha before incubation with endothelial monolayers. Additionally, the extent of binding of antiphospholipid antibodies to intact and damaged endothelium was studied by immunofluorescent techniques and enzyme-linked immunosorbent assay. Our results indicate that prostacyclin production is not impaired by sera containing antiphospholipid antibodies, regardless of the condition of the endothelium. Further, we were unable to demonstrate binding of immunoglobulin from sera containing antiphospholipid antibodies to intact...Continue Reading

References

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Citations

Feb 1, 1996·American Journal of Obstetrics and Gynecology·V C ZammitM A Brown
Apr 13, 2001·European Journal of Obstetrics, Gynecology, and Reproductive Biology·T K Eskes
Apr 1, 1990·Baillière's Clinical Rheumatology·D J Dudley, D W Branch
Jun 1, 1993·Australian and New Zealand Journal of Medicine·M A Kirkland, A S Gallus
Dec 1, 1991·American Journal of Obstetrics and Gynecology·R K SilverJ R Hageman
Apr 20, 2011·The Journal of Obstetrics and Gynaecology Research·Go IchikawaManami Suzuki
Mar 1, 1992·Prostaglandins, Leukotrienes, and Essential Fatty Acids·D W BranchM D Mitchell
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Sep 1, 1992·Baillière's Clinical Obstetrics and Gynaecology·D A Triplett
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Dec 17, 2008·Women's Health·Jeffrey M DenneyRobert L Goldenberg
Dec 10, 1999·Hypertension in Pregnancy : Official Journal of the International Society for the Study of Hypertension in Pregnancy·E D GalleryS Campbell

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Antiphospholipid Syndrome

Antiphospholipid syndrome or antiphospholipid antibody syndrome (APS or APLS), is an autoimmune, hypercoagulable state caused by the presence of antibodies directed against phospholipids.