PMID: 7536841Jan 1, 1995Paper

Pathophysiology of congenital diaphragmatic hernia. X: Localization of nitric oxide synthase in the intima of pulmonary artery trunks of lambs with surgically created congenital diaphragmatic hernia

Journal of Pediatric Surgery
H L KaramanoukianR G Azizkhan

Abstract

The pathophysiology of congenital diaphragmatic hernia (CDH) results from a combination of pulmonary hypoplasia, pulmonary hypertension, and surfactant deficiency. Previously we demonstrated that inhaled nitric oxide (NO), a known vasodilator, only improves oxygenation and decreases pulmonary artery pressures when the lamb model of CDH is pretreated with exogenous surfactant. Nitric oxide synthase (NOS) in endothelial cells is responsible for the production of NO, a mediator of smooth muscle cell relaxation. Pulmonary hypertension in CDH may result from a defect in the endogenous production of NO. Our aim was to determine whether the main pulmonary artery trunks in CDH lambs have NOS immunoreactivity. Cryostat sections of paraformaldehyde-fixed specimens of pulmonary artery and aortic rings from 10 CDH lambs and five control lambs were processed for NADPH-diaphorase activity. Immunolocalization of NOS was studied in paraformaldehyde-fixed sections and compared with serially cut specimens from identical rings that were tested for NADPH-diaphorase activity. Intense NADPH-diaphorase staining was present in the intimal layer (endothelial lining) of the pulmonary artery and aortic rings of both the CDH and control lambs. This activi...Continue Reading

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Citations

Jul 1, 1997·Pediatric Surgery International·M S IrishP L Glick
Oct 28, 1998·Archives de pédiatrie : organe officiel de la Sociéte française de pédiatrie·B ThébaudJ C Mercier
Jul 2, 2002·American Journal of Respiratory Cell and Molecular Biology·Bernard ThébaudA Tuan Dinh-Xuan
Aug 1, 1996·Journal of Pediatric Surgery·H L KaramanoukianP L Glick
Oct 17, 1998·Pediatric Pulmonology·H IJsselstijn, D Tibboel
Jul 17, 2003·Pediatric Case Reviews·H L Hedrick
Apr 11, 2003·Pediatric and Developmental Pathology : the Official Journal of the Society for Pediatric Pathology and the Paediatric Pathology Society·Monique E De PaepeFrancois I Luks
Oct 26, 2005·Journal of Gastroenterology and Hepatology·Rahim AghazadehFarzad Firouzi
Nov 4, 2000·Journal of Gastroenterology and Hepatology·S K YangY K Kim
Apr 27, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·B ThébaudA T Dinh-Xuan
Nov 30, 2010·Experimental and Molecular Pathology·Beena G SoodRaja Rabah
Nov 18, 2004·Journal of Pediatric Surgery·Christopher E ColbyUNKNOWN Congenital Diaphragmatic Hernia Study Group
Jul 19, 2005·American Journal of Surgery·John R GoscheScott C Boulanger

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