Pathophysiology of LPS-induced gastrointestinal injury in the rat: role of secretory phospholipase A2

Shock
Mayssa ZayatElizabeth J Dial

Abstract

A hydrophobic layer of phosphatidylcholine (PC) overlies and protects the surface of the gastrointestinal (GI) tract, contributing to barrier integrity. During critical illness such as sepsis, gut barrier integrity is compromised, which could be related to degradation of PC. The purpose of this study was to investigate a role for luminal (secretory) phospholipase A2 (sPLA(2)) in LPS-induced GI injury. Rats were treated with LPS (5 mg/kg) or saline for 0.5, 1, 3, and 5 h. The gastric and ileal luminal contents were collected for determination of sPLA(2) activity, and the luminal lipids were analyzed using thin layer chromatography for lyso-PC content. The GI permeability was assessed in vivo with fluorescein-isothiocyanate dextran 4000 and rats were tested with or without a specific sPLA(2) inhibitor. LPS induced significant increases in sPLA(2) activity and lyso-PC content in the gastric and ileal lumens at 5 h. In addition, LPS treated rats showed a significant increase in GI permeability to fluorescein-isothiocyanate dextran in both the stomach and ileum at 5 h, which was prevented by pretreatment with the sPLA(2) inhibitor. In response to LPS, sPLA(2) activity increases in the GI tract lumen where it may degrade the extracel...Continue Reading

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Citations

Jun 18, 2009·Critical Care Medicine·Rongqian WuPing Wang
Nov 2, 2011·JPEN. Journal of Parenteral and Enteral Nutrition·Joseph F PierreKenneth A Kudsk
Sep 7, 2017·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·E F CollaresC B Collares-Buzato
May 18, 2010·Shock·Ahmed M Al-MousawiDavid N Herndon
Jul 17, 2008·Shock·Stephen F Lowry
Dec 4, 2009·Current Opinion in Critical Care·Sonja Fruhwald, Johann Kainz
Jul 3, 2010·Biochimica Et Biophysica Acta·Robert EhehaltWolfgang Stremmel

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