Pathotypes of bovine verotoxigenic Escherichia coli isolates producing attaching/effacing (AE) lesions in the ligated intestinal loop assay in rabbits

Advances in Experimental Medicine and Biology
B ChinaJacques Mainil

Abstract

Effacement of the microvilli and intimate attachment to the enterocytes (AE lesions) are two common properties of enteropathogenic (EPEC) and many verotoxigenic (VTEC) E. coli isolates from humans and animals. However not all of the several chromosomal and plasmidic genes and loci involved in the pathogenesis of the human EPEC strain E2348/69 are present in EPEC and VTEC isolates from animal species. We here report that in addition to verotoxin-encoding genes, bovine VTEC isolates harbour a variant of the original eaeA gene, confirming previous results, but neither the eaf nor the hfp loci which are involved in early attachment stage, and that not all of them possess an eaeB gene, as determined by the colony hybridization assay. Have these bovine VTEC isolates lost some of the loci or are they not necessary for the production of AE lesions in vivo? We also report the results of the ligated intestinal loop assay in rabbits with several bovine VTEC isolates. The production of AE lesions was correlated with the presence of an eaeA gene, but not with the presence of an eaeB gene, and was of course independent of the presence of the eaf and bfp loci. The eaeA-negative VTEC isolates produced no AE lesions. Either the eaeB gene is unn...Continue Reading

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.