PMID: 827826Dec 1, 1976

Pathway of testosterone biosynthesis in the testis of the marmoset Saguinus oedipus

J P Preslock, E Steinberger


These studies were undertaken to determine the principal pathway of androgen biosynthesis by the testis of the marmoset Saguinus oedipus. Testicular fragments (25 mg) were incubated at 37 degrees C in Krebs-Ringer bicarbonate buffer, pH 7.4, containing pregnenolone-7-3H (3beta-hydroxy-5-pregnen-20-one) or progesterone-7-3H. Duplicate fragments were incubated with each substrate for 30 min, one hr, three hr, or five hr, for a total of 16 separate incubations. Metabolites were separated by paper and thin-layer chromatography, with identity established by recrystallization to constant specific activities and 3H/14C ratios. Pregnenolone was readily metabolized to progesterone, 17alpha-hydroxyprogesterone, androstenedione (4-androstene-3, 17-dione) and testosterone. Progesterone was converted to 17alpha-hydroxyprogesterone, androstenedione and testosterone. 17alpha-hydroxyprogesterone was the predominant metabolite obtained from both substrates at one, three and five hrs of incubation. Neither 17alpha-hydroxypregnenolone (3beta-17-dihydroxy-5-pregnen-20-one) nor dehydroepiandrosterone (3beta-hydroxy-5-androsten-17-one) was detected in the incubates. These data suggest a predominant delta4 pathway with accumulation of 17alpha-hydroxy...Continue Reading


Feb 1, 1974·Endocrinology·J G SowellK B EIK-NES
Dec 1, 1965·Steroids·W R Slaunwhite, M J Burgett
Mar 1, 1967·Endocrinology·D C SharmaR I DORFMAN
Jul 1, 1967·Steroids·J C Hoschoian, A C Brownie
Apr 1, 1969·Biology of Reproduction·C B Metz
Jan 1, 1970·Folia primatologica; international journal of primatology·P Hershkovitz
Jan 1, 1970·The Journal of Clinical Endocrinology and Metabolism·G B SerraR B Jaffe
Nov 15, 1963·Biochimica Et Biophysica Acta·K B EIK-NES, M KEKRE


Feb 1, 1978·Journal of Steroid Biochemistry·J P Preslock, E Steinberger
Aug 1, 1979·Journal of Steroid Biochemistry·J P Preslock, E Steinberger
Aug 1, 1980·Journal of Steroid Biochemistry·J P Preslock
Sep 1, 1978·Steroids·J P Preslock, E Steinberger
Jan 1, 1985·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·H InanoB Tamaoki
Dec 1, 1977·General and Comparative Endocrinology·J P Preslock, E Steinberger

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