Pathway optimization by re-design of untranslated regions for L-tyrosine production in Escherichia coli

Scientific Reports
Seong Cheol KimGyoo Yeol Jung

Abstract

L-tyrosine is a commercially important compound in the food, pharmaceutical, chemical, and cosmetic industries. Although several attempts have been made to improve L-tyrosine production, translation-level expression control and carbon flux rebalancing around phosphoenolpyruvate (PEP) node still remain to be achieved for optimizing the pathway. Here, we demonstrate pathway optimization by altering gene expression levels for L-tyrosine production in Escherichia coli. To optimize the L-tyrosine biosynthetic pathway, a synthetic constitutive promoter and a synthetic 5'-untranslated region (5'-UTR) were introduced for each gene of interest to allow for control at both transcription and translation levels. Carbon flux rebalancing was achieved by controlling the expression level of PEP synthetase using UTR Designer. The L-tyrosine productivity of the engineered E. coli strain was increased through pathway optimization resulting in 3.0 g/L of L-tyrosine titer, 0.0354 g L-tyrosine/h/g DCW of productivity, and 0.102 g L-tyrosine/g glucose yield. Thus, this work demonstrates that pathway optimization by 5'-UTR redesign is an effective strategy for the development of efficient L-tyrosine-producing bacteria.

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Citations

Mar 22, 2016·ACS Synthetic Biology·Manish KushwahaAlfonso Jaramillo
Jan 28, 2017·Biotechnology for Biofuels·Dong Hun KwakGyoo Yeol Jung
Jun 22, 2018·Applied Microbiology and Biotechnology·Jingyu WangKechun Zhang
Nov 6, 2018·Journal of Industrial Microbiology & Biotechnology·Jillian M HagelPeter J Facchini
Jan 5, 2017·Microbial Cell Factories·Yelin ZhuJiong Hong
Jan 4, 2020·The Journal of Microbiology·Jun RenDokyun Na
Mar 28, 2020·Proceedings of the National Academy of Sciences of the United States of America·Xiaoqiang MaKang Zhou
Jul 10, 2020·Journal of Industrial Microbiology & Biotechnology·Zhu LiDawei Zhang
Apr 16, 2020·Biotechnology and Applied Biochemistry·Rajesh K SrivastavaSarat Babu Imandi
Nov 1, 2020·Applied Microbiology and Biotechnology·Xiaolei GuoJianzhong Huang
Nov 18, 2020·Applied Microbiology and Biotechnology·Wen-Hai XieLi-Juan Wang

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Methods Mentioned

BETA
PCR
Protein Assay
liquid column chromatography

Software Mentioned

UTR Designer
UTR Library Designer
RBS Calculator

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