Pathway-specific reporter genes to study stem cell biology.

Stem Cells
Karen M PetersonMartin Rodriguez-Porcel

Abstract

Little is known on the phenotypic characteristics of stem cells (SCs) after they are transplanted to the myocardium, in part due to lack of noninvasive platforms to study SCs directly in the living subject. Reporter gene imaging has played a valuable role in the noninvasive assessment of cell fate in vivo. In this study, we validated a pathway-specific reporter gene that can be used to noninvasively image the phenotype of SCs transplanted to the myocardium. Rat mesenchymal SCs (MSCs) were studied for phenotypic evidence of myogenic characteristics under in vitro conditions. After markers of myogenic characteristics were identified, we constructed a reporter gene sensor, comprising the firefly luciferase (Fluc) reporter gene driven by the troponin T (TnT) promoter (cardio MSCs had threefold expression in polymerase chain reaction compared to control MSCs) using a two-step signal amplification strategy. MSCs transfected with TnT-Fluc were studied and validated under in vitro conditions, showing a strong signal after MSCs acquired myogenic characteristics. Lastly, we observed that cardio MSCs had higher expression of the reporter sensor compared to control cells (0.005 ± 0.0005 vs 0.0025 ± 0.0008 Tnt-Fluc/ubiquitin-Fluc, P < .05),...Continue Reading

References

Mar 4, 2000·The New England Journal of Medicine·S H LeeP A Thistlethwaite
Dec 6, 2001·Proceedings of the National Academy of Sciences of the United States of America·M IyerS S Gambhir
Mar 12, 2003·Genes & Development·Tarik F Massoud, Sanjiv S Gambhir
Aug 6, 2004·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·Joseph C WuSanjiv S Gambhir
Aug 23, 2005·Molecular Therapy : the Journal of the American Society of Gene Therapy·Martin Rodriguez-PorcelSanjiv Sam Gambhir
Feb 28, 2006·Nature Clinical Practice. Cardiovascular Medicine·Atta Behfar, Andre Terzic
Sep 23, 2006·The New England Journal of Medicine·Volker SchächingerUNKNOWN REPAIR-AMI Investigators
Feb 22, 2008·Nature·Vincent F M Segers, Richard T Lee
Oct 7, 2008·Journal of Molecular and Cellular Cardiology·Atta BehfarAndre Terzic
Jun 13, 2009·Journal of the American College of Cardiology·James S ForresterEduardo Marbán
Dec 17, 2009·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Martin Rodriguez-PorcelSanjiv S Gambhir
Apr 29, 2010·Current Cardiology Reports·Martin Rodriguez-Porcel
May 7, 2013·JACC. Cardiovascular Imaging·Peter J PsaltisMartin Rodriguez-Porcel
Aug 14, 2013·Human Gene Therapy Methods·Ian Y ChenSanjiv S Gambhir

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Citations

Sep 24, 2020·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Sung-Bae Kim, Ramasamy Paulmurugan
Mar 5, 2021·Current Protocols·Karen M PetersonMartin Rodriguez-Porcel
Oct 23, 2020·Biotechnology Journal·Sean D MadsenKim C O'Connor

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