Pathways for the influx of molecules into cercariae of Schistosoma mansoni during skin penetration

Parasitology
J A ThornhillJ R Kusel

Abstract

It has been observed that fluorescent membrane-impermeant molecules can enter the cercariae as they penetrate mouse skin. The hypothesis to be tested was that such molecules, which included Lucifer Yellow and a variety of fluorescent dextrans, entered the parasite through the nephridiopore and excretory tubules as well as through the surface membrane. FITC-labelled poly-L-lysine (molecular weight 10 kDa), added at 4 degrees C during syringe transformation, was found to enter the nephridiopore and labelled the excretory bladder and sometimes the excretory tubules. This finding indicates that macromolecules (10 kDa) can enter the nephridiopore. It was found that linoleic acid (a normal constituent of skin) greatly stimulated uptake of Lucifer Yellow and dextrans into the excretory/subtegumental region of 2-h-old schistosomula. This correlated with an increased uptake of membrane-impermeant propidium iodide at 37 degrees C. Since increased uptake of propidium iodide occurs when membranes become permeable, the surface membrane could also be a pathway of transport of the membrane-impermeant molecules into the schistosomulum.

References

Jul 1, 1988·Molecular and Biochemical Parasitology·J T JonesJ R Kusel
Nov 1, 1989·International Journal for Parasitology·J T JonesJ R Kusel
May 1, 1974·Biological Reviews of the Cambridge Philosophical Society·R A Wilson, L A Webster
Jan 1, 1973·International Journal for Parasitology·D J Hockley, D J McLaren
Apr 1, 1993·Kidney International·J K Leypoldt, L W Henderson
Dec 18, 2001·Micron : the International Research and Review Journal for Microscopy·Charles H DorseyMargaret A Stirewalt
Jan 30, 2002·The Journal of General Physiology·Virginijus Valiunas
Nov 27, 2002·Applied and Environmental Microbiology·M Graça da SilveiraTjakko Abee
Jan 28, 2003·Current Opinion in Critical Care·Nicholas E Vlahakis, Rolf D Hubmayr
Aug 30, 2003·EMBO Reports·Mattias GoksörThomas Nyström
Apr 21, 2004·Critical Reviews in Biomedical Engineering·Frederick H Silver, Lorraine M Siperko
Jan 1, 2005·Theriogenology·Lindsay GillanW M C Maxwell
Nov 5, 2005·Molecular & Cellular Proteomics : MCP·Simon Braschi, R Alan Wilson
Dec 1, 2006·Advances in Parasitology·Patrick J Skelly, R Alan Wilson
May 17, 2007·Nature Reviews. Microbiology·Ian R BoothSamantha Miller
Oct 20, 2007·Conference Proceedings : ... Annual International Conference of the IEEE Engineering in Medicine and Biology Society·Michelle C LaPlacaHillary R Irons
Dec 25, 2007·Parasitology Research·Simone Haeberlein, Wilfried Haas
Jul 1, 2008·Experimental Parasitology·Wilfried HaasEveline Zoppelli
Aug 23, 2008·Prostaglandins & Other Lipid Mediators·Vanessa H OliveiraJulio A Mignaco

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