Pathways of retinoid synthesis in mouse macrophages and bone marrow cells

Journal of Leukocyte Biology
Haixia NiuJ S Welch

Abstract

In vivo pathways of natural retinoid metabolism and elimination have not been well characterized in primary myeloid cells, even though retinoids and retinoid receptors have been strongly implicated in regulating myeloid maturation. With the use of a upstream activation sequence-GFP reporter transgene and retrovirally expressed Gal4-retinoic acid receptor α in primary mouse bone marrow cells, we identified 2 distinct enzymatic pathways used by mouse myeloid cells ex vivo to synthesize retinoic acid receptor α ligands from free vitamin A metabolites (retinyl acetate, retinol, and retinal). Bulk Kit(+) bone marrow progenitor cells use diethylaminobenzaldehyde-sensitive enzymes, whereas bone marrow-derived macrophages use diethylaminobenzaldehyde-insensitive enzymes to synthesize natural retinoic acid receptor α-activating retinoids (all-trans retinoic acid). Bone marrow-derived macrophages do not express the diethylaminobenzaldehyde-sensitive enzymes Aldh1a1, Aldh1a2, or Aldh1a3 but instead, express Aldh3b1, which we found is capable of diethylaminobenzaldehyde-insensitive synthesis of all trans-retinoic acid. However, under steady-state and stimulated conditions in vivo, diverse bone marrow cells and peritoneal macrophages showed...Continue Reading

References

Apr 1, 1991·Journal of Cellular Physiology·P E StenbergL Corash
Dec 17, 1987·Nature·V GiguereR M Evans
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·G AllenbyP Chambon
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·R W StormsC Smith
Aug 10, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·S TakeshitaA Kudo
Oct 19, 2000·The Journal of Biological Chemistry· van Bennekum AMW S Blaner
Nov 13, 2001·Oncogene·P Kastner, S Chan
Jan 6, 2004·The Journal of Nutrition·Harold C Furr
Jan 7, 2004·The Journal of Experimental Medicine·Yoshihiro UedaGarnett Kelsoe
Jan 24, 2004·Leukemia & Lymphoma·Tal OrenTodd Evans
Sep 6, 2005·Experimental Hematology·Todd Evans
Sep 22, 2005·The Journal of Biological Chemistry·Limin Liu, Lorraine J Gudas
Oct 26, 2005·The Biochemical Journal·Caroline E GrahamMartin J Warren
May 10, 2006·The Journal of Experimental Medicine·Louise E PurtonPierre Chambon
Jul 22, 2006·Proceedings of the National Academy of Sciences of the United States of America·John P ChuteDonald P McDonnell
Mar 27, 2007·Biochemical and Biophysical Research Communications·Satori A MarchittiVasilis Vasiliou
Feb 16, 2008·PPAR Research·Eimear M MullenAustin J Cooney
Apr 16, 2008·Analytical Biochemistry·Maureen A KaneJoseph L Napoli
Jul 10, 2008·Expert Opinion on Drug Metabolism & Toxicology·Satori A MarchittiVasilis Vasiliou
Nov 26, 2008·The Journal of Biological Chemistry·Michelle Y S ShihRobert V Farese
May 20, 2009·The Journal of Clinical Investigation·Jacqueline E PaytonTimothy J Ley
Aug 12, 2010·Free Radical Biology & Medicine·Satori A MarchittiVasilis Vasiliou
Aug 24, 2010·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Satori A MarchittiVasilis Vasiliou
Dec 8, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Tamás RoszerMercedes Ricote
Mar 3, 2011·The Journal of Clinical Investigation·John S WelchTimothy J Ley
Mar 26, 2011·The Journal of Clinical Investigation·Lukas D WartmanTimothy J Ley

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.