PMID: 8946635Nov 1, 1996Paper

Patient-administered tampon-collected genital cells in the assessment of Chlamydia trachomatis infection using polymerase chain reaction

Sexually Transmitted Diseases
S N TabriziS M Garland

Abstract

Diagnosis of genital Chlamydia trachomatis infection in women traditionally requires a speculum examination to collect endocervical cells, followed by cell culture. This method is time consuming, requires stringent transport conditions, and is technically demanding. To compare tampons as a patient-administered collection method followed by detection with polymerase chain reaction (PCR) with the traditional endocervical swab culture followed by cell culture detection. At the emergency department of a hospital for obstetrics and gynecology, 1,000 consecutive women with symptoms suggestive of infection with C. trachomatis were tested for C. trachomatis infection by PCR on both tampon (PCR-T) and swab (PCR-S) specimen and by culture of the swab specimen. Seventeen PCR-T and 16 PCR-S specimens were positive; 16 endocervical specimens were positive by culture, and 14 of the endocervical samples were positive by the three methods. Sixty-one PCR-S samples were inadequate as shown by the lack of amplification of the beta-globin gene segment, indicating poor collection of specimens by endocervical swab for chlamydial testing. Tampon specimens collected for PCR detection provided an easy and sensitive method of detection of C. trachomatis...Continue Reading

References

Jun 1, 1992·The Journal of Infectious Diseases·C K FairleyS M Garland
Jun 1, 1991·Genitourinary Medicine·D Taylor-Robinson, B J Thomas
Jul 1, 1988·Journal of Clinical Microbiology·M I LeesS M Garland
Apr 1, 1995·Journal of Clinical Microbiology·M BassiriP A Mårdh
Oct 1, 1994·Journal of Clinical Microbiology·J SchachterH H Lee
Jul 19, 1993·The Medical Journal of Australia·S M GarlandJ A McInnes
Jun 1, 1993·Journal of Clinical Microbiology·R WarrenA M Baker
Mar 1, 1993·Journal of Clinical Microbiology·B J DilleL G Birkenmeyer

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Citations

May 22, 2001·AIDS Patient Care and STDs·F J BowdenS M Garland
Mar 8, 2013·Future Microbiology·Jane S HockingSepehr N Tabrizi
Jun 10, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·S M GarlandSepehr N Tabrizi
Jun 18, 2002·The Medical Journal of Australia·Francis J BowdenChristopher K Fairley

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