Patient-derived xenograft models for pancreatic adenocarcinoma demonstrate retention of tumor morphology through incorporation of murine stromal elements

The American Journal of Pathology
Daniel DelittoJose G Trevino

Abstract

Direct implantation of viable surgical specimens provides a representative preclinical platform in pancreatic adenocarcinoma. Patient-derived xenografts consistently demonstrate retained tumor morphology and genetic stability. However, the evolution of the tumor microenvironment over time remains poorly characterized in these models. This work specifically addresses the recruitment and incorporation of murine stromal elements into expanding patient-derived pancreatic adenocarcinoma xenografts, establishing the integration of murine cells into networks of invading cancer cells. In addition, we provide methods and observations in the establishment and maintenance of a patient-derived pancreatic adenocarcinoma xenograft model. A total of 25 histologically confirmed pancreatic adenocarcinoma specimens were implanted subcutaneously into nonobese diabetic severe combined immunodeficiency mice. Patient demographics, staging, pathological analysis, and outcomes were analyzed. After successful engraftment of tumors, histological and immunofluorescence analyses were performed on explanted tumors. Pancreatic adenocarcinoma specimens were successfully engrafted in 15 (60%) of 25 attempts. Successful engraftment does not appear to correlate...Continue Reading

References

Jun 1, 1997·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·H A BurrisD D Von Hoff
Nov 4, 2004·JAMA : the Journal of the American Medical Association·Thomas G RobertsJeffrey W Clark
Aug 11, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Belen Rubio-ViqueiraManuel Hidalgo
Apr 25, 2007·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Malcolm J MooreUNKNOWN National Cancer Institute of Canada Clinical Trials Group
May 13, 2011·The New England Journal of Medicine·Thierry ConroyUNKNOWN PRODIGE Intergroup
Apr 18, 2012·Nature Reviews. Clinical Oncology·John J TentlerS Gail Eckhardt
Oct 18, 2013·The New England Journal of Medicine·Daniel D Von HoffMarkus F Renschler
Jan 9, 2014·CA: a Cancer Journal for Clinicians·Rebecca SiegelAhmedin Jemal
Apr 18, 2014·Genome Medicine·Raquel Martinez-GarciaManuel Hidalgo

❮ Previous
Next ❯

Citations

Feb 2, 2016·Cancer Treatment Reviews·Sandra SchmitzJean-Pascal Machiels
May 3, 2016·Trends in Cancer·Lindsey A BakerDavid A Tuveson
Jan 21, 2017·Nature Reviews. Cancer·Annette T ByrneLivio Trusolino
Oct 28, 2017·Nature Reviews. Cancer·Nicolas GengenbacherHellmut G Augustin
May 9, 2018·Cancer Science·Hiroyoshi Y Tanaka, Mitsunobu R Kano
Nov 23, 2016·Disease Models & Mechanisms·Milcah C ScottJaime F Modiano
Aug 17, 2018·Pancreas·Sally E HendersonThomas A Mace
Aug 17, 2018·Transplantation·Florence RenaudFrançois Pattou
Feb 13, 2019·Cells·Karl Roland EhrenbergClaudia R Ball
Feb 3, 2016·Molecules and Cells·Sung-Yup ChoHansoo Park
Nov 15, 2019·Chinese Medical Journal·Cheng-Fang Wang, Xian-Jie Shi
Feb 11, 2020·Journal of Cachexia, Sarcopenia and Muscle·Rachel L NosackaAndrew R Judge
Apr 17, 2020·Physiological Reviews·Elisabeth HessmannAlbrecht Neesse
Dec 3, 2016·Oncotarget·Daniel DelittoJose G Trevino
Jun 25, 2020·Japanese Journal of Clinical Oncology·Yanmei ZhangWei Xu
May 18, 2019·Archives of Pathology & Laboratory Medicine·Kenneth P H Pritzker, Heikki J Nieminen
Feb 11, 2020·Frontiers in Oncology·Mirna SwaydenJuan Iovanna
Feb 14, 2019·Journal of Gastrointestinal Surgery : Official Journal of the Society for Surgery of the Alimentary Tract·Matthew C HernandezMark J Truty
May 20, 2020·Therapeutic Advances in Medical Oncology·Tianqi SuJiannan Li
Dec 6, 2018·Nature Communications·Aslamuzzaman KaziSaïd M Sebti
Jun 23, 2019·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Aslamuzzaman KaziSaid M Sebti
Nov 8, 2020·Biochimica Et Biophysica Acta. Reviews on Cancer·Haidi ChenShunrong Ji
Jan 23, 2021·Scientific Reports·John KokkinosPhoebe A Phillips
Dec 18, 2020·Frontiers in Oncology·Gioacchino P MarcecaCarlo M Croce
Jan 17, 2019·Cell Reports·Ellen M LangerRosalie C Sears

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.