Patient-derived xenograft mouse models of pseudomyxoma peritonei recapitulate the human inflammatory tumor microenvironment

Cancer Medicine
Murali R KurachaVenkatesh Govindarajan

Abstract

Pseudomyxoma peritonei (PMP) is a neoplastic syndrome characterized by peritoneal tumor implants with copious mucinous ascites. The standard of care for PMP patients is aggressive cytoreductive surgery performed in conjunction with heated intraperitoneal chemotherapy. Not all patients are candidates for these procedures and a majority of the patients will have recurrent disease. In addition to secreted mucin, inflammation and fibrosis are central to PMP pathogenesis but the molecular processes that regulate tumor-stromal interactions within the peritoneal tumor microenvironment remain largely unknown. This knowledge is critical not only to elucidate PMP pathobiology but also to identify novel targets for therapy. Here, we report the generation of patient-derived xenograft (PDX) mouse models for PMP and assess the ability of these models to replicate the inflammatory peritoneal microenvironment of human PMP patients. PDX mouse models of low- and high-grade PMP were generated and were of a similar histopathology as human PMP. Cytokines previously shown to be elevated in human PMP were also elevated in PDX ascites. Significant differences in IL-6 and IL-8/KC/MIP2 were seen between human and PDX ascites. Interestingly, these cytoki...Continue Reading

References

Aug 1, 1974·American Journal of Obstetrics and Gynecology·G B Feldman, R C Knapp
Aug 7, 2002·The American Journal of Pathology·Jerome T O'ConnellSanford H Barsky
Feb 1, 2003·Biochemical and Biophysical Research Communications·Hiroshi YamamotoYasuhito Yuasa
Jun 15, 2004·Journal of the American College of Surgeons·Rony A Adam, Yehuda G Adam
Jan 29, 2010·BMC Developmental Biology·Daniel BurgessVenkatesh Govindarajan
Jul 1, 2010·International Journal of Experimental Pathology·Arun A MavanurJon M Davison
Apr 21, 2011·Current Oncology·F MohamedP Sugarbaker
Dec 17, 2011·Cancer Cell·Michele W L TengMark J Smyth
Mar 24, 2012·Cancer Cell·Douglas Hanahan, Lisa M Coussens
May 16, 2012·Journal of Surgical Oncology·Haroon A ChoudryDavid L Bartlett
May 23, 2012·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Terence C ChuaDavid L Morris
Aug 1, 2012·Oncology Letters·Mingli LiuJonathan K Stiles
Sep 29, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Jamie L McClellanE Angela Murphy
Mar 2, 2013·Nature Reviews. Drug Discovery·Douglas W McMillinConstantine S Mitsiades
Jun 5, 2013·Cancer Research·Despina Siolas, Gregory J Hannon
Sep 21, 2013·Annals of Surgical Oncology·Kush LohaniBrian Loggie
Nov 14, 2013·ISRN Oncology·Dominique GalesTemesgen Samuel
Mar 13, 2014·Seminars in Immunology·Qing ChangJacqueline Bromberg
Jul 6, 2014·Cold Spring Harbor Protocols·Leonard D ShultzDale L Greiner
Jan 16, 2015·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Jean-François RossiBernard Klein

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Methods Mentioned

BETA
xenograft
xenografts
xenografting

Software Mentioned

GraphPad
GraphPad Prism

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