patS minigenes inhibit heterocyst development of Anabaena sp. strain PCC 7120

Journal of Bacteriology
Xiaoqiang WuJames W Golden

Abstract

The patS gene encodes a small peptide that is required for normal heterocyst pattern formation in the cyanobacterium Anabaena sp. strain PCC 7120. PatS is proposed to control the heterocyst pattern by lateral inhibition. patS minigenes were constructed and expressed by different developmentally regulated promoters to gain further insight into PatS signaling. patS minigenes patS4 to patS8 encode PatS C-terminal 4 (GSGR) to 8 (CDERGSGR) oligopeptides. When expressed by P(petE), P(patS), or P(rbcL) promoters, patS5 to patS8 inhibited heterocyst formation but patS4 did not. In contrast to the full-length patS gene, P(hepA)-patS5 failed to restore a wild-type pattern in a patS null mutant, indicating that PatS-5 cannot function in cell-to-cell signaling if it is expressed in proheterocysts. To establish the location of the PatS receptor, PatS-5 was confined within the cytoplasm as a gfp-patS5 fusion. The green fluorescent protein GFP-PatS-5 fusion protein inhibited heterocyst formation. Similarly, full-length PatS with a C-terminal hexahistidine tag inhibited heterocyst formation. These data indicate that the PatS receptor is located in the cytoplasm, which is consistent with recently published data indicating that HetR is a PatS ta...Continue Reading

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Citations

Sep 29, 2011·Biochemistry·Erik A FeldmannMichael A Kennedy
Dec 8, 2009·Nature Reviews. Microbiology·Enrique Flores, Antonia Herrero
Nov 10, 2009·Proceedings of the National Academy of Sciences of the United States of America·Douglas D Risser, Sean M Callahan
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May 15, 2013·Molecular Microbiology·Laura Corrales-GuerreroAntonia Herrero
Jan 5, 2006·Molecular Microbiology·Cheng-Cai ZhangSylvie Bédu
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May 11, 2016·Proceedings of the National Academy of Sciences of the United States of America·Javier Muñoz-García, Saúl Ares
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Feb 6, 2017·Proceedings of the National Academy of Sciences of the United States of America·Zhenggao ZhengJindong Zhao

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