Pattern recognition receptor responses in children with chronic hepatitis B virus infection

Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology
Ida Louise HeibergBirthe Hogh

Abstract

Several studies have demonstrated that hepatitis B virus (HBV) affects the expression and function of Toll like receptors (TLRs), but data on TLR function in HBV infection are mainly from adult patients. The natural history of chronic hepatitis B (CHB) infection is distinctly different in children, since 90% of children become chronic carriers compared to 5% of adults when infected with HBV. We wanted to study the function of TLRs and cytosolic DNA receptors in children with CHB infection compared to healthy children. PBMCs from 19 children with CHB and 19 healthy children were stimulated with ligands for TLR 2, 3, 4, 7 and 9 for 24 h. For activation of cytosolic DNA receptors, cells were transfected with a double-stranded DNA using Lipofectamine 2000. Supernatants were analyzed for levels of IFN-α, TNF-α, IL-6, CXCL10 and CCL3 by Luminex. Stimulation with ligands for TLR2, TLR3 and TLR9 induced IL-6, CCL3 and CXCL10 to a significantly higher level in children with CHB compared to healthy children. CHB patients displayed significantly lower IFN-α production compared to healthy children after stimulation with ligands for TLR2, TLR3 and TLR4. Stimulation of intracellular DNA sensors with synthetic double-stranded DNA elicited sig...Continue Reading

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Citations

Jul 12, 2014·Viral Immunology·Mohammad Zare-BidakiMohammad Kazemi Arababadi
Jan 31, 2014·Molecular Biology Reports·Masoud Karimi-Googheri, Mohammad Kazemi Arababadi

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