Pattern-recognition receptors in duck (Anas platyrhynchos): identification, expression and function analysis of toll-like receptor 3.

British Poultry Science
Tiantian GuLizhi Lu

Abstract

1. Innate immunity provides the first line of defence against pathogenic organisms through a myriad of germline encoded receptors called pattern-recognition receptors (PRRs). Toll-like receptor (TLR) 3, as an important member of PRRs, is indispensable for host defence against viral infection by recognising virus-derived RNAs. However, little is known about the structure and function of TLR3 in ducks (Anas platyrhynchos), a natural host for the avian influenza virus.2. This study cloned the full-length cDNA of duck TLR3 using reverse transcription polymerase chain reaction (RT-PCR) with rapid amplification of cDNA ends (RACE). The cDNA sequence of duck TLR3 was 4046 bp in length and encoded 895 amino acids. Multiple sequence alignment showed that duck TLR3 shared high similarity with that from other vertebrates.3. Quantitative real-time PCR (qRT-PCR) analysis suggested that TLR3 mRNA was constitutively expressed in all tissues tested, having higher levels in the kidney, liver, breast muscle, ovary and heart. After stimulation with viral- or bacterial-mimics, including LPS, poly(I:C), pam3CSK4, FLS-1, FLA-ST and R848, the TLR3 transcript was significantly upregulated. Meanwhile, overexpression of duck TLR3 significantly promoted ...Continue Reading

References

Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 28, 2002·Annual Review of Immunology·Charles A Janeway, Ruslan Medzhitov
Jan 14, 2003·Annual Review of Immunology·Kiyoshi TakedaShizuo Akira
Oct 14, 2003·Trends in Immunology·Jessica K BellDavid M Segal
Jul 2, 2004·Nature Reviews. Immunology·Shizuo Akira, Kiyoshi Takeda
Jul 16, 2004·Journal of Virology·Karl W Boehme, Teresa Compton
Sep 30, 2004·Nature Immunology·Akiko Iwasaki, Ruslan Medzhitov
Jan 22, 2005·Veterinary Immunology and Immunopathology·Muhammad IqbalAdrian L Smith
Jun 14, 2005·Current Opinion in Immunology·Taro Kawai, Shizuo Akira
Jan 18, 2006·Cell Death and Differentiation·T Kawai, S Akira
Feb 14, 2006·Cell Research·Prafullakumar TailorKeiko Ozato
Feb 25, 2006·Cell·Shizuo AkiraOsamu Takeuchi
Sep 18, 2007·Developmental and Comparative Immunology·Adam J KarpalaAndrew G Bean
Nov 6, 2007·International Immunology·Takahiro SugiyamaTsuneyasu Kaisho
Apr 8, 2009·Molecular and Cellular Biology·Pierre GéninAhmet Civas
Aug 19, 2009·Biochemical and Biophysical Research Communications·Himanshu KumarShizuo Akira
May 21, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Susan E SweeneyGary S Firestein
Oct 19, 2010·Avian Pathology : Journal of the W.V.P.A·Pete Kaiser

❮ Previous
Next ❯

Software Mentioned

Clustal W
MEGA
BioEdit Sequence Alignment Editor
DNAstar
ProtParam
ExPASy

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Alejandra Garcia-CattaneoPhilippe Benaroch
The Journal of Immunology : Official Journal of the American Association of Immunologists
Xiaoyan XiaoLi Wen
Current Research in Translational Medicine
F Morinet
© 2021 Meta ULC. All rights reserved