Abstract
Lamivudine treatment of individuals with chronic HBV infection leads to a rapid decline of hepatitis B virus (HBV) serum DNA. Because HBV replication quickly reaches pretreatment values following cessation of the drug, we addressed the question of whether changes during therapy in composition and amount of discernible circulating viral DNA and RNA might provide an explanation for this phenomenon. Nucleic acids were extracted from serial serum samples of two chronically infected patients. The first patient was treated with lamivudine for 14 weeks, whereas the second one, who displayed an HBV virus with a core gene mutation, received lamivudine for 10 weeks. Three sequence segments of the HBV genome synthesized successively during replication, namely, X, C, and X-preC, were analyzed via competitive polymerase chain reaction (PCR) and reverse transcriptase (RT)/PCR. HBV transcripts were also analyzed for differential polyadenylation. At the start of treatment, identical DNA copy numbers (10(9)/mL) were found for all three segments in the first patient. C segment DNA displayed the expected rapid decline. X-preC, a target contiguous only on plus-strand DNA, behaved similarly. In contrast, the X segment DNA copy numbers showed a less...Continue Reading
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Citations
Oct 8, 2008·Annals of the New York Academy of Sciences·Wei ZhangYing-Ming Feng
Oct 8, 2008·Annals of the New York Academy of Sciences·Ying-Hsiu SuTimothy M Block
Oct 5, 2018·Journal of Virology·Lu BaiZhenghong Yuan
Oct 27, 2018·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Shi LiuHaitao Guo
Dec 18, 2018·Frontiers in Physiology·Jean-Eric BlatteauNicolas Vallée
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Feb 23, 2020·Journal of Hepatology·Bernd StadelmayerFabien Zoulim
May 7, 2021·PeerJ·Pakkapon RattanachaisitPiyawat Komolmit