Patterns of endogenous gangliosides and metabolic processing of exogenous gangliosides in cerebellar granule cells during differentiation in culture

Neurochemical Research
L RiboniG Tettamanti

Abstract

The qualitative and quantitative pattern of endogenous gangliosides and the routes of metabolic processing of exogenous GM1, 3H labeled in the sphingosine moiety (Sph-3H GM1) were studied in cerebellar granule cells during differentiation in vitro. During the first 7-8 days in culture the ganglioside content markedly increased, and the qualitative pattern showed, in percentage terms, a drastic decrease of GD3 and a marked increase of GD2, O-Ac-GT1b, O-Ac-GQ1b and GQ1b. After pulse with (Sph-3H) GM1, at all the investigated days in culture, different radiolabelled lipids were formed indicating that taken up exogenous GM1 was degraded and that its catabolic fragments, and partly GM1 itself, were used for biosynthetic purposes; moreover radioactive water was measured in the culture medium during chase indicating that labelled sphingosine underwent also degradation. The uptake of exogenous GM1 and the extent of its metabolic processing per cell unit increased during differentiation: a) GM2 was the major metabolic product and was relatively more abundant at 2 than 7 days in culture; b) the percentage of metabolites of biosynthetic origin over total metabolites increased during differentiation, especially at the short pulse times; c)...Continue Reading

References

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Citations

Oct 1, 1995·Molecular and Chemical Neuropathology·F Omodeo-SaléP Palestini
Apr 7, 2005·Parasitology Research·Helen Pope-DelatorreLouis N Irwin
Sep 11, 2008·Glycoconjugate Journal·Alessandro PrinettiSandro Sonnino
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Feb 6, 2020·International Journal of Molecular Sciences·Elena ChiricozziLaura Mauri
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