PMID: 7622040Jul 15, 1995Paper

Patterns of gene action in plant development revealed by enhancer trap and gene trap transposable elements

Genes & Development
Venkatesan SundaresanR Martienssen

Abstract

The crucifer Arabidopsis thaliana has been used widely as a model organism for the study of plant development. We describe here the development of an efficient insertional mutagenesis system in Arabidopsis that permits identification of genes by their patterns of expression during development. Transposable elements of the Ac/Ds system carrying the GUS reporter gene have been designed to act as enhancer traps or gene traps. A novel selection scheme maximizes recovery of unlinked transposition events. In this study 491 plants carrying independent transposon insertions were generated and screened for expression patterns. One-half of the enhancer trap insertions and one-quarter of the gene trap insertions displayed GUS expression in seedlings or flowers, including expression patterns specific to organs, tissues, cell types, or developmental stages. The patterns identify genes that act during organogenesis, pattern formation, or cell differentiation. Transposon insertion lines with specific GUS expression patterns provide valuable markers for studies of Arabidopsis development and identify new cell types or subtypes in plants. The diversity of gene expression patterns generated suggests that the identification and cloning of Arabido...Continue Reading

Citations

Apr 8, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·M BevanR McCombie
Jul 26, 2006·Plant Cell Reports·Burgund M BassunerEdward C Yeung
Jul 19, 2008·Molecular Genetics and Genomics : MGG·Michael A GbadegesinJohn R Beeching
Feb 8, 2011·Sexual Plant Reproduction·Stefano BencivengaSimona Masiero
Oct 12, 2005·Plant Molecular Biology·Gynheung AnSichul Lee
Aug 10, 2006·Plant Molecular Biology·Audrey CreffThierry Desnos
Apr 13, 2007·Plant Molecular Biology·Michael A AyliffeAnthony J Pryor
Jul 6, 2007·Plant Molecular Biology·Sung Han ParkChang-Deok Han
Oct 2, 2009·Plant Molecular Biology·Xi CaoDe Ye
Jun 7, 2008·Journal of Biosciences·Virupapuram VijaybhaskarImran Siddiqi
Apr 7, 2000·Journal of Biotechnology·R WambuttM Rose
Feb 13, 2001·Plant Science : an International Journal of Experimental Plant Biology·L ØstergaardJ Mundy
Jun 22, 2001·Trends in Genetics : TIG·E J Finnegan
Jun 4, 1999·Trends in Genetics : TIG·M Bevan, G Murphy
May 14, 1999·Trends in Plant Science·T MaesT Gerats
Mar 14, 2000·Current Opinion in Plant Biology·V Walbot
Feb 27, 1999·Current Opinion in Plant Biology·K Schneitz
Jan 1, 2000·Molecular Plant Pathology·J E ParkerJ D Jones
Jul 29, 1998·The Plant Journal : for Cell and Molecular Biology·J MathurZ Koncz-Kálmán
Jul 29, 1998·The Plant Journal : for Cell and Molecular Biology·V KirikH Bäumlein
May 7, 1999·The Plant Journal : for Cell and Molecular Biology·L CampisiT Jack
Jul 27, 1999·The Plant Journal : for Cell and Molecular Biology·H NaestedJ Mundy
Oct 3, 1999·The Plant Journal : for Cell and Molecular Biology·H G ChinC D Han
Jul 8, 2000·The Plant Journal : for Cell and Molecular Biology·J S JeonG An
Sep 6, 2000·The Plant Journal : for Cell and Molecular Biology·B DubreucqL Lepiniec
Feb 24, 2001·The Plant Journal : for Cell and Molecular Biology·J C KimM J Cho
Sep 5, 2001·The Plant Journal : for Cell and Molecular Biology·X LiY Zhang
Nov 7, 2001·The Plant Journal : for Cell and Molecular Biology·Y HiwatashiM Hasebe
Jul 9, 2002·The Plant Journal : for Cell and Molecular Biology·Kathrin SchrickGerd Jürgens
Oct 18, 2002·The Plant Journal : for Cell and Molecular Biology·Gabino RíosCsaba Koncz
Jan 22, 2003·The Plant Journal : for Cell and Molecular Biology·Julien CurabaGilles Vachon
Feb 6, 2010·Proceedings of the National Academy of Sciences of the United States of America·Takashi KuromoriKazuo Shinozaki

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.