Apr 29, 2020

The Arabidopsis mitochondrial dicarboxylate carrier 2 maintains leaf metabolic homeostasis by uniting malate import and citrate export

BioRxiv : the Preprint Server for Biology
C. P. LeeA. Harvey Millar

Abstract

Malate is the major substrate for respiratory oxidative phosphorylation in illuminated leaves. In the mitochondria malate is converted to citrate either for replenishing tricarboxylic acid (TCA) cycle with carbon, or to be exported as substrate for cytosolic biosynthetic pathways or for storage in the vacuole. In this study, we show that DIC2 functions as a mitochondrial malate/citrate carrier in vivo in Arabidopsis. DIC2 knockout (dic2-1) results in growth retardation that can only be restored by expressing DIC2 but not its closest homologs DIC1 or DIC3, indicating that their substrate preferences are not identical. Malate uptake by non-energised dic2-1 mitochondria is reduced but can be restored in fully energised mitochondria by altering fumarate and pyruvate/oxaloacetate transport. A reduced citrate export but an increased citrate accumulation in substrate-fed, energised dic2-1 mitochondria suggest that DIC2 facilitates the export of citrate from the matrix. Consistent with this, metabolic defects in response to a sudden dark shift or prolonged darkness could be observed in dic2-1 leaves, including altered malate, citrate and 2-oxoglutarate utilisation. There was no alteration in TCA cycle metabolite pools and NAD redox sta...Continue Reading

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Mentioned in this Paper

Visual System
Blood - Brain Barrier Anatomy
Entire Lateral Geniculate Body
Patterns
Cortex Bone Disorders
Adrenal Cortex Diseases
Magnetic Resonance Imaging
Research Subject
Dorsal Nucleus of Lateral Geniculate Body
Anatomic Structures

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