PMID: 6160258Aug 1, 1980Paper

Patterns of transcription of human cytomegalovirus in permissively infected cells

Journal of Virology
J M DeMarchiA S Kaplan

Abstract

The rate of accumulation of cytomegalovirus transcripts in permissively infected human embryonic lung (HEL) cells was analyzed at various times after infection by hybridization of infected cell RNA to undigested or restriction endonuclease-digested cytomegalovirus DNA fixed to nitrocellulose filters. Differences in patterns of transcript accumulation were determined by measuring the abundance levels of RNA which hybridized to different HindIII-, XbaI-, or EcoRI-generated fragments of cytomegalovirus DNA. The results showed that a small but significant amount of cytomegalovirus RNA was detectable within the first 3 h after infection and that the rate of accumulation of these transcripts was static during the first 24 h, but increased thereafter. In general, the viral transcripts accumulating in infected cells could be divided into three classes. Immediate-early RNA (synthesized in the absence of protein synthesis in infected cells) hybridizes predominantly to a very restricted part of the genome and can be identified during the first 2 to 4 h postinfection. Early RNA (synthesized up to about 24 h after infection) originates from most regions of the genome but is characterized by the presence of transcripts which hybridize in gre...Continue Reading

References

Feb 1, 1975·Journal of Virology·S TanakaS A Plotkin
Jan 1, 1975·Proceedings of the Society for Experimental Biology and Medicine·T FurukawaS A Plotkin
Jun 1, 1976·Journal of Virology·J M DeMarchi, A S Kaplan
Sep 1, 1977·The Journal of General Virology·S Michelson-FiskeH Febvre
Dec 15, 1977·Nature·S Michelson-FiskeJ C Guillon
Jan 1, 1979·Microbiology and Immunology·S TanakaY Watanabe
Dec 1, 1973·Journal of Virology·E S HuangJ S Pagano
Nov 1, 1970·Proceedings of the Society for Experimental Biology and Medicine·B B Wentworth, L French
Jun 1, 1973·Journal of Virology·A FiorettiS A Plotkin
Jun 15, 1974·International Journal of Cancer. Journal International Du Cancer·W HenleG Henle
Jun 13, 1966·Biochemical and Biophysical Research Communications·D T Denhardt
Feb 1, 1974·Journal of Virology·S C JeorF Rapp

❮ Previous
Next ❯

Citations

Sep 6, 2005·Journal of Virology·Finn GreyJay Nelson
Mar 2, 2012·Microbiology and Immunology·Yejin KwonChang-Yong Cha
Oct 20, 2019·Vaccines·Mario Alberto Ynga-DurandLuka Cicin-Sain
Jan 1, 2000·Archives of Pharmacal Research·J J HyunH J Kim
Oct 17, 1998·Methods : a Companion to Methods in Enzymology·C Söderberg-NauclérJ A Nelson
Jan 1, 1984·Journal of Virology·R M StenbergM F Stinski
Dec 1, 1985·Journal of Virology·R M Stenberg, M F Stinski

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.