PAX6 Isoforms, along with Reprogramming Factors, Differentially Regulate the Induction of Cornea-specific Genes.

Scientific Reports
Yuzuru SasamotoKohji Nishida

Abstract

PAX6 is the key transcription factor involved in eye development in humans, but the differential functions of the two PAX6 isoforms, isoform-a and isoform-b, are largely unknown. To reveal their function in the corneal epithelium, PAX6 isoforms, along with reprogramming factors, were transduced into human non-ocular epithelial cells. Herein, we show that the two PAX6 isoforms differentially and cooperatively regulate the expression of genes specific to the structure and functions of the corneal epithelium, particularly keratin 3 (KRT3) and keratin 12 (KRT12). PAX6 isoform-a induced KRT3 expression by targeting its upstream region. KLF4 enhanced this induction. A combination of PAX6 isoform-b, KLF4, and OCT4 induced KRT12 expression. These new findings will contribute to furthering the understanding of the molecular basis of the corneal epithelium specific phenotype.

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Citations

Nov 22, 2016·Journal of Cellular Physiology·Jacqueline Martínez-RendónRefugio García-Villegas
Jan 19, 2018·Stem Cells and Development·Jung Woo ParkRen-He Xu
Feb 24, 2018·Expert Opinion on Biological Therapy·Yuzuru SasamotoNatasha Y Frank
Sep 13, 2019·Journal of Cellular and Molecular Medicine·Laura TomaselloCarla Giordano
Jan 19, 2018·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Qingbing MengMinqian Zheng
Dec 22, 2019·Genes·Dulce Lima CunhaMariya Moosajee
Jan 14, 2020·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Anna-Klara RubelowskiNóra Szentmáry
Nov 7, 2017·Wiley Interdisciplinary Reviews. Developmental Biology·Gabriel GonzalezNatasha Y Frank
Oct 26, 2020·Experimental Eye Research·María Teresa Ortiz-MeloFederico Castro-Muñozledo
Jan 20, 2021·Nature Communications·Mingsen LiHong Ouyang
Feb 13, 2021·Genesis : the Journal of Genetics and Development·Mohd Tayyab Adil, Jonathan J Henry
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Jun 9, 2021·The Ocular Surface·Ursula Schlötzer-SchrehardtBerthold Seitz

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Datasets Mentioned

BETA
DRA002960

Methods Mentioned

BETA
co-immunoprecipitation
RNA-seq
reverse
Assay
electrophoresis

Software Mentioned

Cufflinks
Fluidigm
R
MATCH
TopHat2
Fluidigm Single - Cell Gene Expression Workflow system
Cytoscape
Bioconductor
TRANSFAC

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