PAX7 promotes CD49f-positive dairy goat spermatogonial stem cells' self-renewal.

Journal of Cellular Physiology
Xiaomin DuJinlian Hua

Abstract

Spermatogenesis is a complex process that originates from and depends on the spermatogonial stem cells (SSCs). The number of SSCs is rare, which makes the separation and enrichment of SSCs difficult and inefficient. The transcription factor PAX7 maintains fertility in normal spermatogenesis in mice. However, for large animals, much less is known about the SSCs' self-renewal regulation, especially in dairy goats. We isolated and enriched the CD49f-positive and negative dairy goat testicular cells by magnetic-activated cell sorting strategies. The RNA- sequencing and experimental data revealed that cells with a high CD49f and PAX7 expression are undifferentiated spermatogonia in goat testis. Our findings indicated that ZBTB16 (PLZF), PAX7, LIN28A, BMPR1B, FGFR1, and FOXO1 were expressed higher in CD49f-positive cells as compared to negative cells and goat fibroblasts cells. The expression and distribution of PAX7 in dairy goat also have been detected, which gradually decreased in testis tissue along with the increasing age. When the PAX7 gene was overexpressed in dairy goat immortal mGSCs-I-SB germ cell lines, the expression of PLZF, GFRα1, ID4, and OCT4 was upregulated. Together, our data demonstrated that there is a subset of s...Continue Reading

References

Mar 1, 1975·Experimental Cell Research·J C Davis, A W Schuetz
Nov 22, 1994·Proceedings of the National Academy of Sciences of the United States of America·R L Brinster, J W Zimmermann
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·T ShinoharaR L Brinster
Sep 11, 1999·Tissue & Cell·L R FrançaH Chiarini-Garcia
Dec 11, 1999·Nucleic Acids Research·M Kanehisa, S Goto
Sep 5, 2003·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Apr 13, 2004·Proceedings of the National Academy of Sciences of the United States of America·Andrew I SuJohn B Hogenesch
May 25, 2004·Nature Genetics·F William BuaasRobert E Braun
Nov 3, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hiroshi KubotaRalph L Brinster
Feb 15, 2005·Developmental Biology·Marie-Claude HofmannMartin Dym
Mar 28, 2006·Nature·Kaomei GuanGerd Hasenfuss
Aug 26, 2006·Nature Clinical Practice. Endocrinology & Metabolism·Hiroshi Kubota, Ralph L Brinster
Dec 11, 2007·Nature Cell Biology·Iain W McKinnellMichael A Rudnicki
Jul 2, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Sridurga Mithraprabhu, Kate L Loveland
Apr 21, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Bart T PhillipsKyle E Orwig
Aug 26, 2011·The Journal of Clinical Investigation·Meredith J GoertzDiego H Castrillon
Dec 14, 2011·Journal of Assisted Reproduction and Genetics·M BahadoraniMohammad H Nasr-Esfahani
Feb 11, 2012·Reproduction in Domestic Animals = Zuchthygiene·F R O de BarrosJ A Visintin
Mar 6, 2012·Cell Stem Cell·Robin M HobbsPier Paolo Pandolfi
Jul 12, 2012·Journal of Assisted Reproduction and Genetics·Banafsheh HeidariBahareh Behzadi
Sep 26, 2013·Proceedings of the National Academy of Sciences of the United States of America·Julia von MaltzahnMichael A Rudnicki
Apr 3, 2014·Journal of Cellular Biochemistry·Haijing ZhuJinlian Hua
Jun 25, 2014·Journal of Assisted Reproduction and Genetics·R Kumar Pramod, Abhijit Mitra
Aug 19, 2014·The Journal of Clinical Investigation·Gina M AloisioDiego H Castrillon
Oct 18, 2015·Nucleic Acids Research·Minoru KanehisaMao Tanabe
Feb 4, 2016·Cell Proliferation·Liming ZhengJinlian Hua
Mar 10, 2016·Seminars in Cell & Developmental Biology·Carla BoitaniElena Vicini

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