PMID: 2118142Sep 1, 1990Paper

Paxillin: a new vinculin-binding protein present in focal adhesions

The Journal of Cell Biology
C E TurnerK Burridge

Abstract

The 68-kD protein (paxillin) is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers in chicken embryo fibroblasts. It is also present in the focal adhesions of Madin-Darby bovine kidney (MDBK) epithelial cells but is absent, like talin, from the cell-cell adherens junctions of these cells. Paxillin purified from chicken gizzard smooth muscle migrates as a diffuse band on SDS-PAGE gels with a molecular mass of 65-70 kD. It is a protein of multiple isoforms with pIs ranging from 6.31 to 6.85. Using purified paxillin, we have demonstrated a specific interaction in vitro with another focal adhesion protein, vinculin. Cleavage of vinculin with Staphylococcus aureus V8 protease results in the generation of two fragments of approximately 85 and 27 kD. Unlike talin, which binds to the large vinculin fragment, paxillin was found to bind to the small vinculin fragment, which represents the rod domain of the molecule. Together with the previous observation that paxillin is a major substrate of pp60src in Rous sarcoma virus-transformed cells (Glenney, J. R., and L. Zokas. 1989. J. Cell Biol. 108:2401-2408), this interaction with vinculin suggests paxillin may be a key component in the control o...Continue Reading

References

Jan 1, 1990·Cell Motility and the Cytoskeleton·M E Groesch, J J Otto
May 1, 1990·The Journal of Cell Biology·G H NuckollsK Burridge
Aug 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·E B PasqualeS J Singer
May 1, 1988·The Journal of Cell Biology·P A Maher, E B Pasquale
Dec 1, 1989·The Journal of Cell Biology·P JonesD R Critchley
Aug 1, 1989·The Journal of Cell Biology·S JakenT Klauck
Oct 23, 1987·Science·E Ruoslahti, M D Pierschbacher
Jan 1, 1987·Annual Review of Cell Biology·C A Buck, A F Horwitz
Mar 1, 1986·The Journal of Cell Biology·J A Wilkins, S Lin
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R HirstL Rohrschneider
Feb 13, 1986·Biochemical and Biophysical Research Communications·D W Litchfield, E H Ball
Apr 1, 1986·The Journal of Cell Biology·J B Meigs, Y L Wang
Jul 31, 1987·Biochemical and Biophysical Research Communications·D H WachsstockS Lin
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·M D Coutu, S W Craig
Nov 1, 1986·The Journal of Cell Biology·M C Beckerle
Oct 1, 1985·The Journal of Cell Biology·B GeigerT Volberg
Aug 5, 1985·Journal of Molecular Biology·L M Milam
Jan 1, 1985·Journal of Cellular Biochemistry·L Molony, K Burridge
Apr 19, 1984·Nature·K Burridge, P Mangeat
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·A Bretscher
May 1, 1963·The Journal of Cell Biology·M G FARQUHAR, G E PALADE

❮ Previous
Next ❯

Citations

Mar 1, 1996·International Journal of Cancer. Journal International Du Cancer·F ImamuraH Akedo
Mar 21, 2000·Journal of Neuroscience Research·A RenaudinL McKerracher
Oct 27, 1997·Journal of Cellular Physiology·Y TsukataniK Takahashi
Dec 1, 1994·Developmental Dynamics : an Official Publication of the American Association of Anatomists·C A BurdsalD R McClay
May 1, 1991·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A Ben-Ze'ev
Jan 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C E Turner
Feb 1, 1997·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S K Hanks, T R Polte
Jan 1, 1991·Cell Motility and the Cytoskeleton·B Geiger, D Ginsberg
Jan 1, 1994·Cell Motility and the Cytoskeleton·J M SangerJ W Sanger
Jan 1, 1995·Cell Motility and the Cytoskeleton·K KatohK Fujiwara
Sep 1, 1991·Journal of Neuroscience Research·E M Abd-el-BassetS Fedoroff
Jul 18, 2002·Cell Biology International·Robert L ShoemanPeter Traub
Jan 1, 1994·Journal of Mathematical Biology·M D Ward, D A Hammer
Sep 1, 1995·Cancer Metastasis Reviews·K MatsumotoR H Kramer
Mar 1, 1994·Cancer Metastasis Reviews·S H Lo, L B Chen
Jan 16, 2007·Journal of Materials Science. Materials in Medicine·V MuhonenJ Tuukkanen
Jul 21, 2006·Molecular and Cellular Biochemistry·Ralitca Valtcheva-SarkerRoumen Pankov
Jun 1, 1993·The International Journal of Biochemistry·E K KirkeeideA Vedeler
Dec 2, 1990·Cell Differentiation and Development : the Official Journal of the International Society of Developmental Biologists·K BurridgeC Turner
Oct 1, 1991·Current Opinion in Cell Biology·D Ingber
Oct 1, 1991·Current Opinion in Cell Biology·C E Turner, K Burridge
Feb 1, 1993·Current Opinion in Neurobiology·J Stappert, R Kemler
Mar 17, 2004·Experimental Cell Research·Victoria C FolettaOra Bernard
Jan 5, 2002·Biomaterials·Sharon L SanbornKandice Kottke-Marchant
Mar 7, 2001·Biology of the Cell·V Petit, J P Thiery
Jun 5, 2003·Matrix Biology : Journal of the International Society for Matrix Biology·Ibrahima DiagneSheila G Haworth
Apr 16, 2002·The International Journal of Biochemistry & Cell Biology·Simona ParisIvan de Curtis
Aug 1, 1997·The International Journal of Biochemistry & Cell Biology·J L Guan

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