Paxillin is tyrosine-phosphorylated by and preferentially associates with the calcium-dependent tyrosine kinase in rat liver epithelial cells.

The Journal of Biological Chemistry
X Li, H S Earp

Abstract

We and others have recently cloned a non-receptor, calcium-dependent tyrosine kinase (CADTK; also known as PYK2, CAKbeta, and RAFTK) that shares both overall domain structure and 45% amino acid identity with p125(FAK). We have studied the signaling, activation, and potential function of these related enzymes in GN4 rat liver epithelial cells that express CADTK and p125(FAK) at roughly similar levels. p125(FAK) is nearly fully tyrosine-phosphorylated in resting GN4 cells. In contrast, while CADTK is not tyrosine-autophosphorylated in untreated cells, angiotensin II increases CADTK Tyr(P) by 5-10-fold. With regard to signaling, CADTK activation is correlated with stimulation of c-Jun N-terminal kinase and p70(S6K) pathways but not with the stimulation of mitogen-activated protein kinase or p90(RSK). In this report we assessed the contribution of CADTK and p125(FAK) to tyrosine phosphorylation of focal contact proteins. In adherent GN4 cells, the constitutive activity of p125(FAK) was correlated with basal paxillin, tensin, and p130(CAS) tyrosine phosphorylation. A rapid increase in the tyrosine phosphorylation of each protein was detected after treatment with angiotensin II or other agonists that stimulate CADTK; the prolonged 3-...Continue Reading

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·W R HuckleH S Earp
Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S K HanksS K Patel
Jun 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M D SchallerJ T Parsons
Dec 1, 1990·Molecular and Cellular Biology·W R HuckleS Earp
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·L J KornbergR L Juliano
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S B KannerJ T Parsons
Sep 1, 1990·The Journal of Cell Biology·C E TurnerK Burridge
Nov 7, 1995·Proceedings of the National Academy of Sciences of the United States of America·T R Polte, S K Hanks
Mar 1, 1994·Molecular and Cellular Biology·M D SchallerJ T Parsons
Jan 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C E Turner
Apr 12, 1994·Proceedings of the National Academy of Sciences of the United States of America·S TanakaK Nagashima
May 1, 1995·Molecular and Cellular Biology·M D Schaller, J T Parsons
Oct 1, 1995·The Journal of Experimental Medicine·K TachibanaC Morimoto
Nov 1, 1995·Molecular and Cellular Biology·I E ZohnH S Earp
Nov 1, 1995·The Journal of Cell Biology·S MiyamotoK M Yamada
Mar 1, 1995·Molecular Biology of the Cell·X Zhu, R K Assoian
Mar 12, 1993·Cell·Z SongyangR J Lechleider
Apr 14, 1995·Science·E A Clark, J S Brugge
Mar 1, 1995·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·A Richardson, J T Parsons
Jan 6, 1995·The Journal of Biological Chemistry·N MorinoY Nojima
Oct 1, 1994·Current Opinion in Cell Biology·M D Schaller, J T Parsons
Nov 1, 1994·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·S H LoL B Chen
Nov 1, 1994·Trends in Biochemical Sciences·S M FellerD Baltimore
Jan 1, 1994·Annual Review of Cell Biology·A Hall
Feb 1, 1993·The Journal of Cell Biology·R L Juliano, S Haskill
Jan 1, 1995·Annual Review of Cell and Developmental Biology·B M JockuschJ Winkler
Jan 1, 1995·Annual Review of Cell and Developmental Biology·M A SchwartzM H Ginsberg

❮ Previous
Next ❯

Citations

Feb 1, 2002·Biochemical and Biophysical Research Communications·Rosemary J KeoghCaroline P D Wheeler-Jones
Jan 15, 2004·Experimental Cell Research·Stéphane DedieuJean-Jacques Brustis
Jun 4, 1999·Progress in Biophysics and Molecular Biology·D D SchlaepferD J Sieg
Nov 4, 2000·Regulatory Peptides·J Haendeler, B C Berk
Jan 8, 1999·Regulatory Peptides·P P SayeskiK E Bernstein
Mar 8, 2000·Cellular Signalling·H AvrahamS Avraham
Oct 20, 2001·Cell Research·D F GuoT Inagami
Nov 7, 2001·The Biochemical Journal·M D Schaller, E M Schaefer
Dec 5, 2002·Cellular Microbiology·Yoshio IshibashiDavid A Relman
Apr 17, 2013·Molecular and Cellular Biology·Inmoo RheeAndré Veillette
Oct 31, 2003·Molecular and Cellular Biology·Yoshihiro TaniyamaKathy K Griendling
Aug 3, 1999·The Journal of Clinical Investigation·M FuortesC Nathan
Jan 24, 2008·Journal of Cardiovascular Pharmacology and Therapeutics·Dale D Tang, Yana Anfinogenova
Jan 1, 2010·Herpesviridae·Jennifer VomaskeDaniel N Streblow
May 16, 2001·Biochemical and Biophysical Research Communications·R W Wrenn
Aug 27, 2013·Cellular and Molecular Life Sciences : CMLS·Radoslav JanoštiakDaniel Rösel
Jun 6, 1998·Proceedings of the National Academy of Sciences of the United States of America·J S FelschE G Peralta
Apr 18, 2000·The Journal of Cell Biology·H UedaJ L Guan
Sep 2, 2014·Journal of Molecular Biology·Murugendra S VanarottiJie J Zheng
Oct 18, 2012·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Michael P Walsh, William C Cole
Aug 26, 2010·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Annemiek BeverdamPeter Koopman
Jul 27, 2014·European Journal of Cell Biology·Radoslav JanoštiakDaniel Rösel
Feb 2, 2010·Journal of Proteomics·Peter C BonnetteLeonard Buckbinder
Apr 14, 2017·Scientific Reports·Jaroslav BranišDaniel Rosel
Jun 5, 2003·Molecular Reproduction and Development·Paolo ChieffiRaffaele Geremia
Mar 7, 2002·Journal of the Renin-angiotensin-aldosterone System : JRAAS·P P Sayeski, K E Bernstein

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.