Pb2+ impairs GABAergic synaptic transmission in rat hippocampal slices: a possible involvement of presynaptic calcium channels

Brain Research
Cheng XiaoDi-Yun Ruan

Abstract

Pb2+ is a common pollutant that causes a wide variety of detrimental effects on developing central nervous system, including cognitive deficit. However, the mechanisms of Pb2+ neurotoxicity remain to be elucidated. GABAergic synaptic transmission in hippocampus is implicated in learning and memory. In the present study, we examined the effects of Pb2+ on GABA(A)-receptor-mediated inhibitory postsynaptic currents (IPSCs), recorded on CA1 pyramidal neurons in rat hippocampal slices, using whole-cell patch clamp recording. Pb2+ significantly inhibited the peak amplitude of evoked IPSCs and increased paired pulse ratio. In addition, Pb2+ (2-50 microM) significantly diminished the frequency of spontaneous IPSCs in a concentration-dependent manner with an IC(50) of 7.56 microM, without changing the amplitude of spontaneous IPSCs. However, Pb2+ (10 microM) did not alter the frequency and amplitude of miniature IPSCs. It was indicated that Pb2+ impaired GABAergic synaptic transmission via a presynaptic mechanism, inhibiting action potential-dependent GABA release. Interestingly, the inhibition of spontaneous IPSC frequency induced by 10 microM Pb2+ was significantly attenuated either in the presence of 100 muM Cd2+ or in a low-calcium ...Continue Reading

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Citations

Nov 3, 2010·Molecular Neurobiology·April P Neal, Tomás R Guilarte
Apr 9, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·April P NealTomás R Guilarte
Jun 20, 2014·Environmental Toxicology·Talia SandersPaul B Tchounwou
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Mar 1, 2013·Toxicology Research·April P Neal, Tomas R Guilarte
Apr 26, 2016·Metallomics : Integrated Biometal Science·Rakshya GorkhaliJenny J Yang
Aug 21, 2020·Environmental Science and Pollution Research International·Ashif IqubalSyed Ehtaishamul Haque
Feb 13, 2021·Ecotoxicology and Environmental Safety·Coline MonchaninMathieu Lihoreau
Feb 3, 2021·Toxics·Daniela Ramírez OrtegaVerónica Pérez de la Cruz
Nov 17, 2021·Applied Biochemistry and Biotechnology·Shrute Kannappan, Bhaskar Chandra Mohan Ramisetty

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