pBECKS. A flexible series of binary vectors for Agrobacterium-mediated plant transformation

Molecular Biotechnology
A C McCormacD F Chen

Abstract

A series of binary T-DNA vectors (pBECKS) has been created for use in the Agrobacterium-mediated genetic transformation of plants. The pBECKS series has corrected the undesirable features of the popular pBIN19 vector; the deleterious mutation within the coding sequence of nptII has been amended and the cloning sites are now adjacent to the right border repeat in order to reduce the possibility of producing truncated sequences of novel genes within transformants. One set of vectors incorporates various combinations of the marker genes gusA, C1/Lc, nptII, hph, and bar, for pursuit of early and stable transformation events. A set of constructs which contain deleted T-DNA borders in various combinations and display predictably altered efficacies for gene transfer has also been created. A modular set of vectors has been designed to facilitate the insertion and transfer of novel gene sequences by providing a nptII-linked plant expression cassette or lacZ-multiple cloning site. A range of antibiotic resistance genes has been incorporated into the non-T-DNA part of the vectors in order to facilitate their selection across the range of Agrobacterium virulence strains.

References

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Citations

Feb 3, 2007·Transgenic Research·Catherine CoutuDwayne D Hegedus
May 18, 2004·Nature Biotechnology·Baoxiu QiColin M Lazarus
Dec 7, 2007·Plant Physiology·Mery Dafny-Yelin, Tzvi Tzfira
Oct 27, 2010·PloS One·Rebecca F MillsLorraine E Williams
May 15, 2008·BioTechniques·William BelknapKent McCue
May 25, 2010·Plant Biotechnology Journal·Oskar SiemianowskiDanuta Maria Antosiewicz
Jan 21, 2004·The Plant Journal : for Cell and Molecular Biology·Agnieszka M MichaleckaAllan G Rasmusson

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