PbGA20ox2 Regulates Fruit Set and Induces Parthenocarpy by Enhancing GA4 Content

Frontiers in Plant Science
Huibin WangLingfei Xu

Abstract

Fruit set and development occur following successful fertilization. Parthenocarpy, a valuable trait in some self-incompatible species, produces seedless fruit without fertilization. Gibberellin (GA) is a crucial hormone in fruit-set regulation and development. While investigating the development of parthenocarpy in pear (Pyrus bretschneideri Rehd.), we determined that GA 20-oxidases (GA20ox) may play key roles in seedless pear fruit development. Sequence analysis revealed three PbGA20ox genes: PbGA20ox1, PbGA20ox2, and PbGA20ox3. We analyzed the expression patterns of candidate genes and found that PbGA20ox2 levels significantly changed in pollinated fruits. Tissue-specific expression assays revealed that PbGA20ox2 is highly expressed in young fruit and leaves. Subcellular localization assays showed it was located in the cytoplasm, nucleus, and plasma membrane. Overexpressed PbGA20ox2 tomato plants were taller and had longer hypocotyls and internodes, and the emasculated flowers produced parthenocarpic fruit. In pear, the transient overexpression of PbGA20ox2 promoted fruit development and delayed the drop of nonpollinated fruit. Furthermore, the fruit of PbGA20ox2-overexpressing tomato and transient PbGA20ox2-overexpressing pe...Continue Reading

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Citations

Jun 8, 2021·Frontiers in Plant Science·Mi HeYanwei Hao

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Methods Mentioned

BETA
PCR
transgenic
Assay
RNA-seq
of

Software Mentioned

ClustalW
Primer Premier
BLASTN
AB StepOne Plus
Bowtie
MEGA

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