PbrmiR397a regulates lignification during stone cell development in pear fruit

Plant Biotechnology Journal
Cheng XueJun Wu

Abstract

Lignified stone cells substantially reduce fruit quality. Therefore, it is desirable to inhibit stone cell development using genetic technologies. However, the molecular mechanisms regulating lignification are poorly understood in fruit stone cells. In this study, we have shown that microRNA (miR) miR397a regulates fruit cell lignification by inhibiting laccase (LAC) genes that encode key lignin biosynthesis enzymes. Transient overexpression of PbrmiR397a, which is the miR397a of Chinese pear (Pyrus bretschneideri), and simultaneous silencing of three LAC genes reduced the lignin content and stone cell number in pear fruit. A single nucleotide polymorphism (SNP) identified in the promoter of the PbrmiR397a gene was found to associate with low levels of fruit lignin, after analysis of the genome sequences of sixty pear varieties. This SNP created a TCA element that responded to salicylic acid to induce gene expression as confirmed using a cell-based assay system. Furthermore, stable overexpression of PbrmiR397a in transgenic tobacco plants reduced the expression of target LAC genes and decreased the content of lignin but did not change the ratio of syringyl- and guaiacyl-lignin monomers. Consistent with reduction in lignin conte...Continue Reading

References

Dec 10, 1998·Nucleic Acids Research·S RombautsP Rouzé
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Michael F. Thomashow
Oct 27, 2004·Trends in Plant Science·Filippo PassardiChristophe Dunand
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·L M LagriminiS Rothstein
Oct 1, 1935·Plant Physiology·W W Smith
Jun 29, 2006·Journal of Experimental Botany·Xiaoning CaiYajun Wu
Sep 26, 2006·The Plant Journal : for Cell and Molecular Biology·Alexandra LanotDianna J Bowles
Jun 19, 2007·Nature Biotechnology·Fang Chen, Richard A Dixon
Jan 16, 2009·Nature Reviews. Molecular Cell Biology·Peter Brodersen, Olivier Voinnet
May 20, 2009·Bioinformatics·Heng Li, Richard Durbin
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Jul 21, 2010·The New Phytologist·Jing-Ke Weng, Clint Chapple
Nov 3, 2010·Nucleic Acids Research·Ana Kozomara, Sam Griffiths-Jones
Mar 25, 2011·Methods in Molecular Biology·Francisco E Nicolas
May 31, 2011·Nucleic Acids Research·Xinbin Dai, Patrick Xuechun Zhao
May 23, 2012·Journal of Agricultural and Food Chemistry·José C del RíoAna Gutiérrez
Nov 15, 2012·Genome Research·Jun WuShaoling Zhang
Feb 13, 2013·Journal of Agricultural and Food Chemistry·Jorge RencoretJosé C del Río
Jun 12, 2013·Proceedings of the National Academy of Sciences of the United States of America·Shanfa LuVincent L Chiang
Oct 23, 2013·Journal of Agricultural and Food Chemistry·Jijiao ZengShulin Chen
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Feb 11, 2015·Plant Physiology·Wu LanJohn Ralph
Feb 18, 2015·The Plant Journal : for Cell and Molecular Biology·Hui ZhouYuepeng Han

❮ Previous
Next ❯

Citations

Nov 13, 2019·Scientific Reports·Prashanti PatelThumballi Ramabhatta Ganapathi
May 24, 2020·Molecular Biology Reports·Tamunonengiyeofori LawsonChiew Foan Chin
Aug 3, 2019·Trends in Plant Science·Marc BehrMarie Baucher
Dec 17, 2020·Journal of Integrative Plant Biology·Nai-Qian Dong, Hong-Xuan Lin
Oct 30, 2020·International Journal of Molecular Sciences·Duc Quan NguyenChristopher P L Grof
Dec 18, 2020·Functional Plant Biology : FPB·Shili HuangYulin Tang
Nov 5, 2021·BMC Plant Biology·Pingping LiShugang Zhao
Jan 16, 2022·Horticulture Research·Jiaming LiJun Wu

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCR
glycosylation
transmission electron microscopy
transfection

Software Mentioned

house Perl script
SAMtools
psRNATarget
SAS
Basic Local Alignment Search Tool ( BLAST )
In
BLAST
MEGA
Trimmomatic
tBLASTn

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.