Pbx4 is Required for the Temporal Onset of Zebrafish Myocardial Differentiation

Journal of Developmental Biology
Robert M KaoLisa Maves

Abstract

Proper control of the temporal onset of cellular differentiation is critical for regulating cell lineage decisions and morphogenesis during development. Pbx homeodomain transcription factors have emerged as important regulators of cellular differentiation. We previously showed, by using antisense morpholino knockdown, that Pbx factors are needed for the timely activation of myocardial differentiation in zebrafish. In order to gain further insight into the roles of Pbx factors in heart development, we show here that zebrafish pbx4 mutant embryos exhibit delayed onset of myocardial differentiation, such as delayed activation of tnnt2a expression in early cardiomyocytes in the anterior lateral plate mesoderm. We also observe delayed myocardial morphogenesis and dysmorphic patterning of the ventricle and atrium, consistent with our previous Pbx knock-down studies. In addition, we find that pbx4 mutant larvae have aberrant outflow tracts and defective expression of the proepicardial marker tbx18. Finally, we present evidence for Pbx expression in cardiomyocyte precursors as well as heterogeneous Pbx expression among the pan-cytokeratin-expressing proepicardial cells near the developing ventricle. In summary, our data show that Pbx4 ...Continue Reading

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Citations

Jan 24, 2017·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Sara E Wirbisky, Jennifer L Freeman
Nov 14, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Justyna Niderla-BieliŃskaAnna Ratajska
Mar 24, 2016·Journal of Developmental Biology·Marine Roux, Stéphane Zaffran
May 1, 2021·Journal of Cardiovascular Development and Disease·Luciana Da Silveira Cavalcante, Shannon N Tessier

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BETA
genotyping
Confocal
confocal microscopy
transgenic

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ImageJ

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