PMID: 11905996Mar 22, 2002Paper

PC12nnr5 cells expressing TrkA receptors undergo morphological but not cholinergic phenotypic differentiation in response to nerve growth factor

Journal of Neurochemistry
Jacqueline C BaskeyR Jane Rylett

Abstract

We investigated mechanisms underlying nerve growth factor-mediated morphological differentiation and expression of cholinergic neuronal phenotype. In PC12, but not PC12nnr5 cells, nerve growth factor induces neurite-like outgrowths and enhances cholinergic phenotype; stable expression of TrkA receptors in nnr5 cells (called B5P cells) restores morphological differentiation but not expression of choline acetyltransferase. Transfection with an AP-1 luciferase reporter gene revealed that PC12 but not B5P cells expressed nerve growth factor-induced functional AP-1 activity. RT-PCR analysis of nerve growth factor-mediated changes in AP-1 transcription factors showed rapid increases in c-fos and junB mRNA in PC12 and B5P cells, while increases in c-jun were small. Using DNA-protein gel shift assays we determined that nerve growth factor stimulates AP-1 binding in both PC12 and B5P cells, and identified c-Fos, FosB, Fra-1, Fra-2, c-Jun, JunB and JunD in AP-1 complexes. In Fos/Jun functional luciferase reporter assays, nerve growth factor stimulated phosphorylation of c-Fos in both PC12 and B5P cells, but phosphorylation of c-Jun only in PC12, and not in B5P cells. These data indicate that mechanisms relating to AP-1 transcription fact...Continue Reading

Citations

Nov 30, 2012·Cellular and Molecular Neurobiology·Tomas R GranaBettina E Kalisch
Oct 20, 2012·BMC Pharmacology & Toxicology·Warren Winick-NgBettina E Kalisch
Nov 19, 2003·Journal of Neurochemistry·Yuko FujiwaraGabor Tigyi
Mar 24, 2015·Neuroscience Letters·Megan R Strachan-WhaleyBettina E Kalisch
Aug 29, 2003·Oncogene·Ljubica IvanisevicH Uri Saragovi

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