PCB 118 and Aryl Hydrocarbon Receptor Immunoassays for Screening Dioxins in Retail Fish

Journal of Agricultural and Food Chemistry
T TsutsumiTamio Maitani

Abstract

The efficacy of a combination of two enzyme-linked immunosorbent assay (ELISA) kits was examined for screening the toxic equivalent (TEQ) concentrations of dioxins in retail fish. The coplanar PCB-EIA system, which is a competitive immunoassay specific for polychlorinated biphenyl (PCB) 118, was tested as a screening method for mono- ortho PCBs. The Ah immunoassay (Ah-I), which is an ELISA-based aryl hydrocarbon receptor binding assay, was analyzed for its screening ability for non- ortho PCBs, polychlorinated dibenzo- p-dioxins (PCDDs), and dibenzofurans (PCDFs). Dilution and recovery tests using purified fish extracts revealed no major interference of the matrix in the PCB-EIA and suggested that the matrix effect was minimized in the Ah-I. Finally, the results for the fish samples ( n = 20) showed a strong correlation between this method and high-resolution gas chromatography coupled to high-resolution mass spectrometry for the determination of the TEQ concentrations of mono- ortho PCBs ( r = 0.99) and non- ortho PCBs and PCDD/Fs ( r = 0.97). These data indicate that our method is suitable for screening retail fish to determine the TEQ concentrations of dioxins.

References

Nov 30, 1998·Environmental Health Perspectives·M Van den BergT Zacharewski
Jun 15, 1999·Food Additives and Contaminants·T F BoveeH A Kuiper
May 12, 2005·Journal of Agricultural and Food Chemistry·Yuki AshizukaTakao Iida
Jul 11, 2006·Toxicological Sciences : an Official Journal of the Society of Toxicology·Martin Van den BergRichard E Peterson
Mar 21, 2007·Shokuhin eiseigaku zasshi. Journal of the Food Hygienic Society of Japan·Tomoaki TsutsumiTamio Maitani

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