PCB congener specific oxidative stress response by microarray analysis using human liver cell line.

Environment International
S DeSisir K Dutta

Abstract

In this study we have examined the effect of exposure to different congeners of PCBs and their role in oxidative stress response. A metabolically competent human liver cell line (HepG2) was exposed with two prototype congeners of PCBs: coplanar PCB-77 and non-coplanar PCB-153. After the predetermined times of exposure (0-24h) at 70 μM concentration, the HepG2 cells showed significant apoptotic changes by fluorescent microscopy after 12h of exposure. Gene set enrichment analysis (GSEA) identified oxidative stress as the predominant enrichment. Further, paraquat assay showed that PCB congeners lead to oxidative stress to different extents, PCB-77 being more toxic. This study, with emphasis on all recommended microarray quality control steps, showed that apoptosis was one of the most significant cellular processes as a result of oxidative stress, but each of these congeners had a unique signature gene expression, which was further validated by Taqman real time PCR and immunoblotting. The pathways involved leading to the common apoptotic effect were completely different. Further in-silico analysis showed that PCB-153 most likely acted through the TNF receptor, leading to oxidative stress involving metallothionein gene families, and...Continue Reading

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Citations

Jan 1, 2014·The Journal of Nutritional Biochemistry·Bradley J NewsomeBernhard Hennig
Oct 12, 2015·Journal of Pharmaceutical and Biomedical Analysis·Sona KrizkovaVojtech Adam
Apr 4, 2015·Toxicology Mechanisms and Methods·Teuta MuratiIvana Kmetič
Oct 28, 2014·Ultrastructural Pathology·Ke SuCheng Chen
Jul 21, 2016·Chemical Research in Toxicology·Esra MutluJames A Swenberg
Jun 27, 2014·Environmental Research·Diego BadernaEmilio Benfenati
Jun 16, 2019·International Journal of Environmental Research and Public Health·Marike M LeijsJens M Baron

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