pCMV-BMP-2-transfected cell-mediated gene therapy in anterior cruciate ligament reconstruction in rabbits

Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association
Ching-Jen WangYu-Lin Yang

Abstract

This study investigated the effect of plasmid cytomegalovirus (pCMV)-bone morphogenetic protein 2 (BMP-2) gene therapy on the healing of the tendon-bone interface after anterior cruciate ligament (ACL) reconstruction in rabbits. The pCMV-BMP-2 was synthesized from full-length human BMP-2 complementary deoxyribonucleic acid, followed by cloning into pCMV Script vector (Clontech Laboratories, Inc., San Jose, CA), and was delivered by a xenogeneic (rat kidney) cell line. The ACL was reconstructed by the transfer of extensor digital tendon in the proximal tibia. In the study group the pCMV-BMP-2 gene-transfected normal rat kidney cells mixed with calcium alginate gel were placed at the tendon-bone interface, whereas no pCMV-BMP-2 was used in the control group. The evaluations included radiography, bone mineral density, magnetic resonance imaging, biomechanical study, histologic examination, and immunohistochemical analysis. Bone mineral density showed no significant difference between the groups (P > .05). Magnetic resonance imaging showed significantly better contact between tendon and bone in the study group compared with the control group (P < .0001). In the biomechanical study, significantly higher failure load and maximal graf...Continue Reading

References

Mar 1, 1988·The American Journal of Sports Medicine·S P ArnoczkyA A Allen
Mar 1, 2005·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Ching-Jen WangYa-Ju Yang
Jan 30, 2007·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Teruo KohnoSatoshi Toh
Mar 24, 2007·The Journal of Clinical Investigation·Christophe DuboisBruce Furie
May 29, 2007·Thrombosis Research·Anna M FallonAjit P Yoganathan

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Citations

Mar 31, 2012·International Orthopaedics·Thomas LaumonierJacques Menetrey
Feb 14, 2012·Cell and Tissue Research·Karen RuschkePetra Knaus
Jul 30, 2014·Current Reviews in Musculoskeletal Medicine·Carlos Eduardo da Silveira FrancioziRene Jorge Abdalla
Nov 13, 2015·Connective Tissue Research·Tim SchwartingPhilipp Lechler
Aug 6, 2014·Artificial Cells, Nanomedicine, and Biotechnology·Dongyang QianYanjun Zeng
Nov 28, 2012·Clinics in Sports Medicine·Bart MullerAsheesh Bedi
Jul 14, 2010·Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association·James H Lubowitz, Gary G Poehling
Feb 9, 2017·Tissue Engineering. Part B, Reviews·Justin L MadrigalEduardo A Silva
Jul 17, 2018·Tissue Engineering. Part a·Wen ZhangElizabeth R Balmayor
Mar 1, 2014·Orthopaedic Journal of Sports Medicine·Sai-Chuen FuKai-Ming Chan
Mar 6, 2021·Bioactive Materials·Tingyun LeiZi Yin
Feb 6, 2013·Orthopedics·Johannes StruewerThomas Manfred Frangen

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