PCNT is critical for the association and conversion of centrioles to centrosomes during mitosis

Journal of Cell Science
Jaeyoun KimKunsoo Rhee

Abstract

A centrosome consists of a pair of centrioles and pericentriolar material (PCM). We manipulated expression of PCNT, a key PCM protein, and investigated roles of PCM in centriole behavior during mitosis. Deletion of PCNT had little effect on interphase centrosomes. However, centrioles in PCNT-deleted mitotic cells prematurely separated and frequently amplified, revealing that centrioles are limited within the spindle poles by PCNT during mitosis. It is known that specific cleavage of PCNT is necessary for centriole separation during mitotic exit. We observed delayed centriole separation in the G0 phase when a non-cleavable mutant form of PCNT was removed or when PCNT was artificially cleaved by TEV protease. Furthermore, a daughter centriole converts to a mother centriole only after experiencing both mitotic exit and specific PCNT cleavage. Based on these results, we propose that a centriole pair disengages upon entering mitosis but remains associated with the surrounding PCM proteins throughout mitosis. During mitotic exit, specific cleavage of PCNT induces PCM disintegration. As a result, a daughter centriole separates from the mother centriole and converts to a young mother centriole.

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Citations

Mar 15, 2020·Biology of the Cell·Véronique Marthiens, Renata Basto
Jul 13, 2019·Cells·Vladimir Joukov, Arcangela De Nicolo
Mar 27, 2020·The Journal of Cell Biology·Nidhi VishnoiC Patrick Lusk
Jan 15, 2021·The Journal of Cell Biology·Takumi ChinenDaiju Kitagawa
Nov 12, 2020·Current Opinion in Structural Biology·Kyung S LeeYan Zeng
Jul 28, 2021·Annual Review of Cell and Developmental Biology·Sónia Gomes PereiraMónica Bettencourt-Dias
Oct 30, 2021·Molecules and Cells·Byungho ShinKunsoo Rhee

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