PCR and dissection as tools to monitor filarial infection of Aedes polynesiensis mosquitoes in French Polynesia

Filaria Journal
Catherine PlichartAnne-Marie Legrand

Abstract

Entomological methods may provide important tools for monitoring the transmission of filariasis in French Polynesia. In order to standardize our PCR method and refine our protocol to assess filarial infection levels in mosquitoes, we compared dissection of the vector, Aedes polynesiensis, with the poolscreening polymerase chain reaction (PS-PCR) assay. (1) Mosquitoes were collected in human landing catches in five areas in Moorea island, French Polynesia. (2) A fraction of the captured mosquitoes was dissected for Wuchereria bancrofti larvae. (3) Laboratory-reared mosquitoes (uninfected as well as experimentally infected ones) were repeatedly tested to optimize a PS-PCR protocol (DNA extracts from 1-50 pooled mosquitoes were tested with an internal standardized system and primers specific for the Ssp1 repeat sequence. PCR products were analysed by gel electrophoresis). (4) Another fraction of the captured mosquitoes was assayed by PS-PCR according the optimized protocol. The prevalence of field-mosquito infection with W. bancrofti ranged from 1 to 8 % by dissection (L1-L3) and point estimates of infection prevalence, as assayed by PS-PCR, ranged from 0.4 to 3.7 %. There was a moderately strong correlation between larval infecti...Continue Reading

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Citations

Nov 13, 2010·Parasitology Research·Darío VezzaniLeonhard Schnittger
Aug 15, 2014·PLoS Neglected Tropical Diseases·Mark A SchmaedickKimberly Y Won
Jun 19, 2008·PLoS Neglected Tropical Diseases·Sandra J LaneySteven A Williams
Dec 21, 2017·PLoS Neglected Tropical Diseases·Djamel TahirPhilippe Parola
Oct 13, 2009·Expert Review of Molecular Diagnostics·Shuzhen SimGeorge Dimopoulos
May 28, 2019·Journal of Vector Ecology : Journal of the Society for Vector Ecology·Matthew M SmithColleen M Handel
Nov 10, 2020·International Journal for Parasitology·Shannon M HedtkeWarwick N Grant

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Methods Mentioned

BETA
MDA
dissection
dissections
PCR
membrane
electrophoresis

Software Mentioned

Poolscreen

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