PCR and microsatellite analysis of diminazene aceturate resistance of bovine trypanosomes correlated to knowledge, attitude and practice of livestock keepers in South-Western Ethiopia

Acta Tropica
Y MotiV Delespaux

Abstract

African Animal Trypanosomosis is threatening the agricultural production and cattle breeding more severely than any other livestock disease in the continent, even more since the advent of drug resistance. A longitudinal study was conducted from November 2012 to May 2013 in the Ghibe valley to evaluate diminazene aceturate (DA) resistance and assess livestock owner's perception of trypanocidal drug use. Four Peasant Associations (PAs) were purposively selected and the cattle randomly sampled in each PAs. At the beginning of the study (t0), 106 bovines positive for trypanosomes by the haematocrit centrifugation technique (HCT) and 119 negative control animals were recruited for six months follow-up using HCT, 18S-PCR-RFLP, DpnII-PCR-RFLP and microsatellite analysis. Prevalence of trypanosomosis was 18.1% based on the HCT technique and the mean PCV value was 23.6±5.1% for the 587 sampled cattle. Out of the 106 HCT positive, 64 (60.4%) were positive for the presence of trypanosomes using the 18S-PCR-RFLP. Species detection showed 38 (59.4%) Trypanosoma congolense savannah, 18 (28.1%) Trypanosoma vivax, 5 (7.8%) Trypanosoma theileri and 3 (4.7%) T. congolense Kilifi. Among the T. congolense savannah samples, 31 (81.6%) showed a DA r...Continue Reading

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Citations

Oct 17, 2015·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·George Laylson da Silva Oliveira, Rivelilson Mendes de Freitas
Oct 11, 2016·Parasitology·Federica GiordaniMichael P Barrett
Aug 2, 2019·The Onderstepoort Journal of Veterinary Research·Harry A MusonyeJames Nonoh
Mar 9, 2021·Veterinary Parasitology : Regional Studies and Reports·Efrem DegnehTekalegn Desta
Apr 29, 2021·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Kayo José Garcia de Almeida Castilho NetoFabiano Antonio Cadioli
Aug 26, 2021·Parasite Epidemiology and Control·Dereje Tulu Robi, Shibiru Diriba

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