PMID: 7541630Jun 1, 1995Paper

PCR and patch-clamp analysis of single neurons

Neuron
Nikolaus J Sucher, D L Deitcher

Abstract

The combination of patch-clamp and molecular biology techniques has made it possible to characterize the pharmacological and biophysical properties of ion channels in single neurons and to screen for expression of specific mRNAs in the same cell. Following whole-cell recording, the cytoplasm of the cell is harvested, and RNA is reverse transcribed into cDNA and amplified in PCR with primers specific for individual ion channel subunits. Additional experiments can then be designed to relate structure and function at the protein level more directly, since cells appear to regulate the composition of ion channels at least partly at the posttranscriptional stage.

References

Aug 1, 1992·Neuron·B LambolezJ Rossier
Jan 1, 1992·Methods in Enzymology·J EberwineR Finnell
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J EberwineP Coleman
Mar 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·R N Van GelderJ H Eberwine
Jul 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·C W LuetjeP Séguéla
Jun 1, 1994·PCR Methods and Applications·W C Gause, J Adamovicz
Dec 1, 1993·PCR Methods and Applications·R S Cha, W G Thilly
Dec 1, 1993·PCR Methods and Applications·C W DieffenbachG S Dveksler
Feb 1, 1994·PCR Methods and Applications·S KwokA Wang

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Citations

Jun 8, 2007·Journal of Molecular Histology·Jean-Claude PlatelAngélique Bordey
Jul 1, 1997·Brain Research. Molecular Brain Research·G SeifertC Steinhäuser
Mar 25, 2000·Neurochemistry International·H K KimelbergM Zhou
Aug 1, 1996·Current Opinion in Cell Biology·S M Assmann, L L Haubrick
Dec 22, 1999·Current Opinion in Biotechnology·T C FreemanP J Richardson
Mar 29, 2001·Autonomic Neuroscience : Basic & Clinical·J K Phillips, J Lipski
Dec 24, 2011·Nature Protocols·Ami CitriRobert C Malenka
Apr 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·K C ChenP W Landfield
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·K TouharaT Haga
Jun 5, 2010·The Journal of Biological Chemistry·Steven B CondliffeMichela Matteoli
Dec 23, 2015·Nature Biotechnology·Cathryn R CadwellAndreas S Tolias
Jul 12, 2013·Annals of the New York Academy of Sciences·Suhasa B KodandaramaiahCraig R Forest
May 9, 2012·Expert Opinion on Biological Therapy·Shan-Li Chen, Jian-Guo Jiang
Jan 10, 2013·FEMS Microbiology Reviews·Paul C Blainey
Jul 6, 2007·Neuron·Shichun TuNobuki Nakanishi
May 28, 2016·International Neurourology Journal·Kieu The Loan TrinhNae Yoon Lee
Aug 21, 2008·PloS One·Daniel J Catron, Mohamed A F Noor
Jan 22, 1996·Proceedings. Biological Sciences·S KorneevJ A Davies
May 10, 2008·Journal of Applied Physiology·Michael J WackerPhilip M Gallagher
Aug 16, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·H YegerE Cutz
Jul 7, 2017·BMC Biology·Cathryn R CadwellAndreas S Tolias
Oct 24, 2018·Frontiers in Molecular Neuroscience·Shreejoy J TripathyPaul Pavlidis
Jul 5, 2005·Neuroscience Letters·Michael J WackerJames A Orr

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