PCR and single-strand conformational polymorphism for recognition of medically important opportunistic fungi.

Journal of Clinical Microbiology
T J WalshS J Chanock

Abstract

The application of PCR technology to molecular diagnostics holds great promise for the early identification of medically important pathogens. PCR has been shown to be useful for the detection of the presence of fungal DNA in both laboratory and clinical samples. Considerable interest has been focused on the utility of selecting universal primers, those that recognize constant regions among most, if not all, medically important fungi. Once an amplicon, or piece of amplified DNA determined by the unique pair of oligonucleotide primers, has been generated, several different methods may be used to distinguish between genera and between species. The two major approaches have utilized differences in restriction enzyme digestion patterns or hybridization with specific probe. We report the application of single-strand conformational polymorphism (SSCP) as a technique to delineate the differences between fungal species and/or genera. Minor sequence variations in small single-stranded DNA cause subtle changes in conformation, allowing these strands to be separated on polyacrylamide gels by SSCP. We used a 197-bp fragment amplified from the 18S rRNA gene, common to all medically important fungi. After amplification, the fragments were den...Continue Reading

References

Feb 1, 1992·Journal of Clinical Microbiology·W G MerzP F Lehmann
Jan 1, 1992·Current Topics in Medical Mycology·M A Pfaller
Jun 21, 1991·Science·H A ErlichJ J Sninsky
Jan 1, 1990·Clinical Microbiology Reviews·J M Jones
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M OritaT Sekiya
Jan 1, 1994·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·T G MitchellW G Merz
Apr 1, 1995·Journal of Clinical Microbiology·S FujitaC J Morrison
Jan 1, 1994·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·M MaiwaldH G Sonntag
Jan 1, 1993·Journal of Medical and Veterinary Mycology : Bi-monthly Publication of the International Society for Human and Animal Mycology·R L HopferW E Highsmith
Jan 1, 1994·Journal of Clinical Microbiology·A R HolmesH F Jenkinson
Sep 1, 1993·The Journal of Infectious Diseases·V L Kan
Mar 1, 1993·Journal of Clinical Microbiology·C SpreadburyJ Cohen

❮ Previous
Next ❯

Citations

Jun 29, 2001·Transplant Infectious Disease : an Official Journal of the Transplantation Society·T J Walsh, A H Groll
Jan 22, 2003·Transplant Infectious Disease : an Official Journal of the Transplantation Society·E L CampagnaroJ A Daller
Feb 18, 2003·Mycoses·Clara Romano, Clelia Miracco
Nov 19, 2003·Current Opinion in Infectious Diseases·Brian L Jones, Lorna A McLintock
Oct 5, 2016·Experimental and Therapeutic Medicine·Alina TanaseSimona Laura Ianosi
Sep 1, 2006·Medical Mycology·P Lewis White, Rosemary A Barnes
Feb 19, 2002·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Elias J AnaissieThomas J Walsh
Jul 5, 2002·Clinical Microbiology Reviews·Siew Fah Yeo, Brian Wong
Sep 11, 2004·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Jong Hee ShinDong Wook Ryang
Jan 31, 2002·World Journal of Gastroenterology : WJG·W Z ZhangS D Zhang
Dec 15, 1996·Nucleic Acids Research·N C NelsonM M Becker
Apr 9, 1999·Medicine·D L Paterson, N Singh
Sep 14, 2004·Revista Do Instituto De Medicina Tropical De São Paulo·Regina Célia PaschoalClaudete Rodrigues Paula
Feb 26, 2010·Nucleosides, Nucleotides & Nucleic Acids·Guofan WuJingwan Kang
Oct 27, 2004·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons
Nov 3, 2001·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·E J AnaissieT J Walsh
Jun 29, 2001·Transplant Infectious Disease : an Official Journal of the Transplantation Society·P E Verweij, J F Meis
Apr 11, 2008·Mycoses·P K ShuklaG B S Keshava
Jun 17, 1998·FEMS Immunology and Medical Microbiology·M E KatzB F Cheetham
Sep 6, 2001·Diagnostic Cytopathology·A E Walts
Apr 9, 2001·Molecular and Cellular Probes·P Cruz-PerezL D Stetzenbach
Oct 7, 2005·Journal of Clinical Microbiology·Dirk M LeinbergerTill T Bachmann
Apr 2, 2003·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C Gil-LamaignereF-M C Müller

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.