PMID: 10747167Apr 4, 2000Paper

PCR assay for species-specific identification of Bacteroides thetaiotaomicron

Journal of Clinical Microbiology
L J TengK T Luh

Abstract

Bacteroides thetaiotaomicron is the second most frequently encountered species of the anaerobes isolated from clinical specimens. We developed a PCR-based assay for the rapid identification of B. thetaiotaomicron. Specific primers were based on shared amplicons of about 1.2 kb generated from B. thetaiotaomicron by randomly amplified polymorphic DNA. This 1.2-kb fragment was sequenced and then used to design a set of PCR amplification primers. This PCR generated an amplification product of 721 bp, which was unique to all 65 isolates of B. thetaiotaomicron tested. There was no amplification with isolates of other bacterial species. Restriction enzyme digestion of the amplification product and dot blot hybridization further verified the specificity of the assay. These results suggest that this PCR assay targets a nucleotide sequence that is strongly conserved in B. thetaiotaomicron. This simple and rapid PCR assay provides a rapid and accurate method for identification of B. thetaiotaomicron and shows promise for the detection of B. thetaiotaomicron in clinical samples.

References

Jun 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·M C RedondoD R Snydman
Jun 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·K Tanaka-BandohK Ueno
May 1, 1995·Journal of Clinical Microbiology·D N WhaleyJ M Miller
Feb 1, 1994·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A ArzeseG A Botta
Mar 1, 1994·Journal of Clinical Microbiology·Y YamashitaM Kaku
Feb 1, 1994·Journal of Bacteriology·B J PasterG J Fraser
May 1, 1996·Letters in Applied Microbiology·T KuwaharaY Ohnishi
Dec 1, 1996·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·D R SnydmanS L Gorbach
Dec 1, 1996·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·E J Goldstein
Oct 6, 1997·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·M C ClarosA C Rodloff
Dec 17, 1997·Journal of Clinical Microbiology·J J CavallaroJ M Miller
Oct 17, 1998·Journal of Clinical Microbiology·F Lo PrestiJ Etienne

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