PMID: 7544866Apr 12, 1995Paper

PCR-based analysis of voltage-gated K+ channels in vascular smooth muscle

Molecular and Cellular Biochemistry
P ZahradkaN Leblanc

Abstract

Irregularities in K+ currents form the basis of several cardiovascular dysfunctions, among which are arrhythmias and vasospasms. The developmental regulation of voltage-gated K+ channels, however, has been difficult to study. A novel approach was therefore employed to examine these channels in muscle tissue. Primers for a PCR-based analysis were designed using published nucleic acid sequences for voltage-gated K+ channels. Final selection of the primer pairs was based on the homology present in the S4 and H5 transmembrane domains. A specific band was amplified with these primers using RNA isolated from both rat A10 vascular smooth muscle cells and rat heart tissue.

References

Mar 15, 1976·Experimental Cell Research·B W Kimes, B L Brandt
Oct 1, 1992·Physiological Reviews·O Pongs
Apr 1, 1992·Computer Applications in the Biosciences : CABIOS·D G HigginsR Fuchs
Sep 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·M PaulmichlD E Clapham
Jul 1, 1990·The American Journal of Physiology·M T NelsonN B Standen
Jan 13, 1989·Cell·L Y Jan, Y N Jan
Jan 1, 1988·Trends in Pharmacological Sciences·N S Cook
May 1, 1983·Journal of Cellular Physiology·P Libby, K V O'Brien
Aug 1, 1993·Cardiovascular Drugs and Therapy·P B BennettM M Tamkun
Jun 1, 1994·The American Journal of Physiology·N LeblancP M Leung
Mar 1, 1993·Circulation Research·G F TomaselliE Marban
Sep 8, 1994·Nature·L Y Jan, Y N Jan
Dec 25, 1993·Nucleic Acids Research·B Fristensky
Oct 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·P J HartB Horowitz

❮ Previous
Next ❯

Citations

Aug 30, 1996·Molecular and Cellular Endocrinology·L Saward, P Zahradka
Jun 5, 2003·Molecular and Cellular Endocrinology·Lorraine Yau, Peter Zahradka
Jan 13, 2006·The Journal of Pharmacology and Experimental Therapeutics·Peter ZahradkaCarla G Taylor

❮ Previous
Next ❯

Related Concepts

Related Feeds

Atrial Fibrillation

Atrial fibrillation is a common arrhythmia that is associated with substantial morbidity and mortality, particularly due to stroke and thromboembolism. Here is the latest research.

Arrhythmia

Arrhythmias are abnormalities in heart rhythms, which can be either too fast or too slow. They can result from abnormalities of the initiation of an impulse or impulse conduction or a combination of both. Here is the latest research on arrhythmias.