PCR-based capsular serotype determination of Haemophilus influenzae strains recovered from Japanese paediatric patients with invasive infection

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
Naruhiko IshiwadaYoichi Kohno

Abstract

The serotypes of 53 isolates of Haemophilus influenzae from children with invasive infections were determined by a conventional slide agglutination test (SAT) and a recently proposed PCR-based method for serotyping H. influenzae. The PCR assay identified 47 (88.7%) type b isolates, one (1.9%) type e isolate and five (9.4%) non-typeable isolates. The only discrepancy between the methods was an isolate that was non-typeable by SAT, but was identified as serotype e by PCR. Of 41 isolates from patients with meningitis, 39 (95.1%) were type b. Of the five non-typeable isolates, three (60%) were from the blood of patients with septicaemic pneumonia and two (40%) were from the cerebrospinal fluid of patients with meningitis. None of the non-typeable isolates appeared to be a capsule-deficient mutant of an encapsulated H. influenzae strain. Overall, the study confirmed the usefulness of this PCR method for the serotyping of invasive H. influenzae isolates.

References

Mar 1, 1979·The Journal of Pediatrics·A S DajaniM C Thirumoorthi
Jun 1, 1992·The Journal of Infectious Diseases·J S Kroll
Oct 1, 1994·Journal of Clinical Microbiology·T J FallaE R Moxon
May 1, 1997·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·A C MoloneyJ M Musser
Feb 12, 1998·The New England Journal of Medicine·A SchuchatB A Perkins
Oct 22, 1998·The Pediatric Infectious Disease Journal·H KamiyaS M Standaert
Jun 26, 2001·The Journal of Infectious Diseases·C OgilvieE E Adderson
May 11, 2002·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Rosemeire C ZanellaMaria Cristina C Brandileone
Jan 9, 2003·Journal of Clinical Microbiology·Leslye L LaClaireUNKNOWN Active Bacterial Core Surveillance Team Members
Dec 10, 2003·Journal of Clinical Microbiology·Sérgio BokermannMaria Cristina de C Brandileone

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Citations

Jun 28, 2005·Antimicrobial Agents and Chemotherapy·Sharon L Bishop-HurleyArnold L Smith
Nov 23, 2006·International Journal of Pediatric Otorhinolaryngology·Dewan S BillalNoboru Yamanaka
Feb 3, 2011·Journal of Infection and Chemotherapy : Official Journal of the Japan Society of Chemotherapy·Takao ItoTsutomu Kamimaki
Oct 14, 2010·International Journal of Medical Microbiology : IJMM·Thiên-Trí LâmHeike Claus
Aug 19, 2010·Kansenshōgaku zasshi. The Journal of the Japanese Association for Infectious Diseases·Kyoko SawadaTadashi Hoshino

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