Abstract
The frequency of the gseA gene encoding a glutamic acid-specific serine protease, GluSE, of Staphylococcus epidermidis was investigated. DNA hybridization analysis demonstrated that gseA existed exclusively in S. epidermidis but not in other bacteria examined. A single step PCR assay with a set of designed primers yielded amplification of gseA from all 69 clinical isolates of S. epidermidis taken from patients and healthy adults, whereas production of GluSE was observed in 74% (51/69) of the isolates. Furthermore, none of the 46 clinical isolates of other species of coagulase-negative staphylococci and 45 clinical isolates of Staphylococcus aureus showed amplification, except a Staphylococcus capitis strain. However, this strain was positive for a S. epidermidis-specific DNA region and the DNA sequence of the 16S rRNA gene showed 99% identity with that of S. epidermidis. Therefore, these results indicated that the present PCR assay for gseA was ubiquitous and highly specific for detection of S. epidermidis.
References
May 22, 1992·Biochimica Et Biophysica Acta·K YoshikawaH Teraoka
Apr 1, 1991·Journal of Clinical Microbiology·W E Kloos, C G George
Jan 1, 1991·Journal of Bacteriology·W G WeisburgD J Lane
Feb 1, 1990·Journal of Bacteriology·A SlomaJ Pero
Jan 1, 1995·Journal of Clinical Microbiology·D L Rhoden, J M Miller
Nov 1, 1994·Canadian Journal of Microbiology·Y Ohara-NemotoM Ota
Dec 2, 1994·Gene·Y SuzukiK Ohsuye
Jan 1, 1994·Diagnostic Microbiology and Infectious Disease·C E GrantJ A Williams
May 13, 1993·Biochimica Et Biophysica Acta·K KitadokoroN Yoshida
Dec 1, 1996·Journal of Clinical Microbiology·F MartineauM G Bergeron
Jan 1, 1997·Journal of Medical Microbiology·S GribaldoJ Stanley
Oct 8, 1997·Journal of Clinical Microbiology·Y Ohara-NemotoM Kaneko
Jan 1, 1997·Microbiology and Immunology·X Hu, K Totake
Mar 12, 1999·Annual Review of Medicine·J Huebner, D A Goldmann
Jul 16, 1999·Journal of Clinical Microbiology·V KrimmerW Ziebuhr
Jun 8, 2000·International Journal of Systematic and Evolutionary Microbiology·M Wieser, H J Busse
Aug 30, 2001·Journal of Clinical Microbiology·K J EdwardsN A Saunders
Apr 5, 2002·Microbes and Infection·Cuong Vuong, Michael Otto
Jul 20, 2002·Microbial Pathogenesis·Yuko Ohara-NemotoShigenobu Kimura
Nov 1, 2002·The Lancet Infectious Diseases·Christof von EiffChristine Heilmann
Citations
Sep 8, 2011·The Journal of Biological Chemistry·Yuko Ohara-NemotoTakayuki K Nemoto
Mar 8, 2005·Journal of Clinical Microbiology·Yuko Ohara-NemotoShigenobu Kimura
Sep 12, 2008·Biological Chemistry·Yuko Ohara-NemotoTakayuki K Nemoto
Aug 17, 2010·Biological Chemistry·Toshio OnoTakayuki K Nemoto
Apr 11, 2014·The Bulletin of Tokyo Dental College·Rie FujiiMasahiro Furusawa
Jul 24, 2007·European Journal of Orthopaedic Surgery & Traumatology : Orthopédie Traumatologie·Fergus J ByrneMichael Kerin
Jun 19, 2013·FEBS Open Bio·Shakh M A RoufTakayuki K Nemoto
Oct 24, 2007·Diagnostic Microbiology and Infectious Disease·Tadayuki IwaseYoshimitsu Mizunoe
Aug 17, 2006·Letters in Applied Microbiology·D LiuM L Lawrence
Aug 7, 2010·Journal of Medical Microbiology·Mitsuo KishiMasami Yonemitsu
Jul 12, 2008·Analytical Biochemistry·Toshio OnoYuko Ohara-Nemoto
Dec 19, 2012·Biochimie·Shakh M A RoufTakayuki K Nemoto
Jan 18, 2014·Pathogens and Disease·Ilse VandecandelaereTom Coenye
Aug 1, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E G A FredheimC Klingenberg
Oct 21, 2016·Antonie van Leeuwenhoek·Chao TanLili Zou
Jan 13, 2018·FEMS Microbiology Letters·Grith M M HermansenLars Jelsbak
Jun 21, 2017·Infection and Immunity·Yuko Ohara-NemotoTakayuki K Nemoto
May 21, 2010·Nature·Tadayuki IwaseYoshimitsu Mizunoe
Jan 9, 2020·Biological Chemistry·Mohammad Tanvir SarwarTakayuki K Nemoto
Feb 6, 2018·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Sergio Martínez-GarcíaJuan C Cancino-Diaz