PCR-based identification of Staphylococcus epidermidis targeting gseA encoding the glutamic-acid-specific protease

Canadian Journal of Microbiology
Y IkedaK Kikuchi

Abstract

The frequency of the gseA gene encoding a glutamic acid-specific serine protease, GluSE, of Staphylococcus epidermidis was investigated. DNA hybridization analysis demonstrated that gseA existed exclusively in S. epidermidis but not in other bacteria examined. A single step PCR assay with a set of designed primers yielded amplification of gseA from all 69 clinical isolates of S. epidermidis taken from patients and healthy adults, whereas production of GluSE was observed in 74% (51/69) of the isolates. Furthermore, none of the 46 clinical isolates of other species of coagulase-negative staphylococci and 45 clinical isolates of Staphylococcus aureus showed amplification, except a Staphylococcus capitis strain. However, this strain was positive for a S. epidermidis-specific DNA region and the DNA sequence of the 16S rRNA gene showed 99% identity with that of S. epidermidis. Therefore, these results indicated that the present PCR assay for gseA was ubiquitous and highly specific for detection of S. epidermidis.

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Citations

Mar 8, 2005·Journal of Clinical Microbiology·Yuko Ohara-NemotoShigenobu Kimura
Aug 17, 2010·Biological Chemistry·Toshio OnoTakayuki K Nemoto
Apr 11, 2014·The Bulletin of Tokyo Dental College·Rie FujiiMasahiro Furusawa
Jul 24, 2007·European Journal of Orthopaedic Surgery & Traumatology : Orthopédie Traumatologie·Fergus J ByrneMichael Kerin
Jan 18, 2014·Pathogens and Disease·Ilse VandecandelaereTom Coenye
Aug 1, 2014·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·E G A FredheimC Klingenberg
Feb 6, 2018·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Sergio Martínez-GarcíaJuan C Cancino-Diaz

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