PCR-based strategy to detect contamination with mycotoxigenic Fusarium species in maize

Systematic and Applied Microbiology
Miguel JuradoM Teresa González-Jaén

Abstract

Contamination of cereals with mycotoxigenic species of Fusarium is an important source of trichothecenes, fumonisins and other mycotoxins which cause serious diseases in human and animals. In addition, these species are phytopathogenic and produce severe losses in cereal yield. Methods for early detection of these Fusarium species are crucial to prevent toxins entering the food chain and are a useful tool in disease management practices. We have developed an integrated protocol for diagnosis of mycotoxigenic Fusarium contamination in maize which can also be used for other cereals. The protocol consisted in an easy and rapid DNA extraction from maize samples (grain and germ), and subsequent group-specific polymerase chain reaction (PCR) assays for genus Fusarium, Gibberella fujikuroi complex, and trichothecene-producing species of Fusarium, that orientate the search of the critical species. We have additionally developed a PCR assay for the identification of F. proliferatum. The primers were designed on the basis of IGS sequence (Intergenic Spacer of rDNA), a multi-copy region in the genome that permits to enhance the sensitivity of the assay in comparison with PCR assays based on single-copy sequences. The suitability of the pr...Continue Reading

References

Jul 16, 2005·Food Additives and Contaminants·G MulèC Waalwijk

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Citations

Mar 26, 2014·Foodborne Pathogens and Disease·Medhat A El-Naggar, Tamer M Thabit
Apr 24, 2012·International Journal of Food Microbiology·Simon G EdwardsMartin C Hare
May 20, 2008·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Amaia González-SalgadoBelén Patiño
May 21, 2010·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Noelia SardiñasBelén Patiño
Nov 20, 2012·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·J Gil-SernaB Patiño
Feb 9, 2012·International Journal of Molecular Sciences·Carla Bertechini FariaIone Parra Barbosa-Tessmann
May 28, 2013·Genome Génome / Conseil National De Recherches Canada·Salvador MireteMaría Teresa González-Jaén
Jan 14, 2012·International Journal of Food Microbiology·Ali AtouiAhmed Lebrihi
Oct 29, 2010·Plant Physiology and Biochemistry : PPB·Elke FouquaertEls J M Van Damme
Nov 17, 2009·Food Microbiology·Miguel JuradoM Teresa González-Jaén
Jul 5, 2011·Journal of the Science of Food and Agriculture·Maiko WatanabeYukiko Hara-Kudo
Apr 29, 2016·Applied Microbiology and Biotechnology·Seon Hwa Kim, Vladimir Vujanovic
Aug 21, 2016·Analytica Chimica Acta·Riikka PeltomaaMaría C Moreno-Bondi
Aug 5, 2017·Journal of Molecular Microbiology and Biotechnology·Adam KuzdralińskiMarta Muszyńska
Jan 29, 2021·Comprehensive Reviews in Food Science and Food Safety·Muiz O AkinyemiChibundu N Ezekiel
Feb 13, 2021·Journal of AOAC International·Ines JedidiMaría T González-Jaén

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