PCR detection assay of fumonisin-producing Fusarium verticillioides strains

Journal of Food Protection
Belén PatiñoCovadonga Vázquez

Abstract

Fusarium verticillioides is considered to be the main source of fumonisins, a group of toxins that contaminate commodities and result in chronic and acute diseases affecting humans and animals. The detection and control of this species is crucial to prevent fumonisins from entering the food chain. The objective of the present research was to develop a specific, sensitive, and robust PCR assay to detect F. verticillioides strains using two pairs of specific primers for F. verticillioides, which have been designed on the basis of the intergenic spacer region of the rDNA units. The first pair of primers was F. verticillioides species specific, whereas the second pair of primers detected fumonisin-producing F. verticillioides strains. This second pair of primers allowed for the discrimination between the major group of F. verticillioides strains, fumonisin-producing strains that are mainly associated with crops, and a minor group of strains, non-fumonisin-producing strains that are associated with bananas. Fifty-four strains of F. verticillioides from different geographical regions and hosts were tested using both sets of primers. Sixteen additional Fusarium species were examined. The specificity of the primer sequences provides th...Continue Reading

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Citations

May 20, 2008·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Amaia González-SalgadoBelén Patiño
Apr 1, 2010·Journal of the Science of Food and Agriculture·Emanuela TorelliEmanuela Gobbi
Aug 27, 2011·Journal of Insect Science·S A PelizzaC E Lange
Nov 20, 2012·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·J Gil-SernaB Patiño
Sep 9, 2020·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·Hamid Ur RahmanPeiwu Li
Apr 16, 2011·Applied Microbiology and Biotechnology·Nayaka S ChandraH S Prakash
Feb 9, 2012·International Journal of Molecular Sciences·Carla Bertechini FariaIone Parra Barbosa-Tessmann
May 25, 2005·FEMS Microbiology Letters·Amaia González-SalgadoM Teresa González-Jaén
Apr 7, 2009·Pest Management Science·Siddaiah Chandra NayakaCarmen N Mortensen
Jun 1, 2007·Canadian Journal of Microbiology·Valéria Nascimento da SilvaBenedito Corrêa
May 28, 2013·Genome Génome / Conseil National De Recherches Canada·Salvador MireteMaría Teresa González-Jaén
Feb 13, 2021·Journal of AOAC International·Ines JedidiMaría T González-Jaén
Nov 17, 2009·Food Microbiology·Miguel JuradoM Teresa González-Jaén

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