PCR detection of Clostridium perfringens producing different toxins in faeces of goats

Letters in Applied Microbiology
F A UzalW R Kelly

Abstract

A polymerase chain reaction (PCR) was used to identify the genes encoding the major toxins of Clostridium perfringens in faeces of goats. When pure cultures of Cl. perfringens types A, B, C, D and E were used as templates in the PCR, amplicons were observed on the agarose gel as bands at approximately the 247 (alpha primers), 1025 (beta primers), 403 (epsilon primers) and 298 (iota primers) bp level of the DNA marker. When used to identify different types of Cl. perfringens in samples artificially spiked with these micro-organisms, the PCR detected as few as 1-1.5 x 10(2) cfu g-1 of the five types of Cl. perfringens tested. The PCR technique allowed the identification and typing of Cl. perfringens strains in faeces of goats, without recourse to other techniques such as the mouse neutralization test.

Citations

Sep 26, 2009·Archives of Microbiology·Luciana A GonçalvesFrancisco C F Lobato
Sep 9, 2004·Microbiology and Molecular Biology Reviews : MMBR·Holger BarthBradley G Stiles
May 17, 2006·Anaerobe·Milton Osmar AguileraAna María Stefanini de Guzmán
Jul 3, 2013·Anaerobe·Ha-Young KimByeong Yeal Jung
Oct 6, 2004·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·F A UzalM Baisón
Aug 23, 2006·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Ken KatsudaHiroshi Tsunemitsu
May 8, 2008·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Francisco A Uzal, J Glenn Songer
Mar 3, 2018·Toxins·Miloslava DuracovaJiri Dresler
Feb 15, 2003·The Veterinary Record·F A UzalM F Miyakawa

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