PMID: 11325547Apr 28, 2001Paper

PCR detection of Helicobacter pylori in string-absorbed gastric juice

FEMS Microbiology Letters
M G Domínguez-BelloP Gueneau de Novoa

Abstract

Molecular methods for detection of Helicobacter pylori infection have been shown to be highly sensitive in gastric biopsies and cultures. The objective of this work was to compare PCR detection of H. pylori DNA in string-absorbed gastric juice and in gastric biopsies. The study was performed in 47 dyspeptic adult patients undergoing endoscopy, and infection was detected by amplification of a segment of H. pylori ureA gene. Of the 29 patients positive in biopsy analysis, 23 (79%) were also positive in the gastric string. PCR analysis of gastric strings is a sensitive and safe procedure to detect H. pylori when endoscopy is not indicated, and may be of great clinical and epidemiological usefulness in determining effectiveness of eradication therapies, typing virulence genes and detecting antibiotic resistance mutations.

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Citations

Sep 28, 2004·Journal of Pediatric Gastroenterology and Nutrition·Tsutomu YamazakiTakaaki Matsumoto
Apr 13, 2007·Clinical Microbiology Reviews·Francis Mégraud, Philippe Lehours
Dec 21, 2012·Journal of Clinical Microbiology·Maria H R B GoncalvesLucia L B C Braga
Jul 22, 2015·The American Journal of Tropical Medicine and Hygiene·Monica ContrerasFabian Michelangeli
Jul 26, 2017·Frontiers in Immunology·Isabelle Laforest-Lapointe, Marie-Claire Arrieta

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