PCR detection of seven virulence and toxin genes of Campylobacter jejuni and Campylobacter coli isolates from Danish pigs and cattle and cytolethal distending toxin production of the isolates

Journal of Applied Microbiology
D D BangM Madsen

Abstract

To study the prevalence of seven virulence and toxin genes, and cytolethal distending toxin (CDT) production of Campylobacter jejuni and C. coli isolates from Danish pigs and cattle. The presence of the cadF, ceuE, virB11, flaA, cdtA, cdtB, cdtC and the cdt gene cluster among 40 C. jejuni and C. coli isolates was detected by polymerase chain reaction. The CDT production of the isolates was determined on Vero, colon 205 and chicken embryo cells. The cadF, flaA, ceuE and cdtB genes were detected from 100% of the isolates. The cdtA and cdtC genes were found in 95.0 and 90.0% of the isolates, respectively. The cdt gene cluster was detected in 82.5% isolates. Only 7.5% of the isolates were positive for virB11. Ninety-five per cent of the isolates produced CDT in Vero and colon 205 cell assays, and 90% of the isolates produced CDT in chicken embryo cell assays. High prevalence of the cadF, ceuE, flaA and cdtB genes was found. Data of the prevalence of cdt genes was consistent with the CDT titres produced by the isolates. Campylobacter coli from pigs produced high CDT titres. The high prevalence of seven virulence and toxin genes demonstrated that these putative pathogenic determinants are widespread among Campylobacter isolates from ...Continue Reading

References

Dec 1, 1977·Infection and Immunity·J KonowalchukS Stavric
Feb 1, 1992·Veterinary Microbiology·B A McFarland, S D Neill
Jul 1, 1992·Journal of Medical Microbiology·I Florin, F Antillon
Feb 1, 1988·Microbial Pathogenesis·W M Johnson, H Lior
Aug 1, 1985·The Journal of Hygiene·P A Manser, R W Dalziel
Jan 1, 1994·Annual Review of Genetics·R R Isberg, G T Van Nhieu
Jun 1, 1993·Journal of Clinical Microbiology·I NachamkinC M Patton
Jan 1, 1997·Microbiology·Julian M Ketley
Jul 1, 1997·Clinical Microbiology Reviews·T M Wassenaar
Oct 10, 1997·FEMS Immunology and Medical Microbiology·E M NielsenM Madsen
May 9, 1998·Infection and Immunity·C A WhitehouseC L Pickett
Nov 11, 1998·Zentralblatt Für Bakteriologie : International Journal of Medical Microbiology·F SchulzeE Borrmann
Jul 3, 1999·Trends in Microbiology·C L Pickett, C A Whitehouse
Feb 23, 2000·BioTechniques·B M MachielsF B Thunnissen
Jul 19, 2000·Infection and Immunity·D J BaconP Guerry

❮ Previous
Next ❯

Citations

Jul 5, 2005·Foodborne Pathogens and Disease·Roger B HarveyDavid J Nisbet
Apr 26, 2008·Journal of Medical Microbiology·Catherine FearnleyDiane G Newell
May 4, 2011·Journal of Food Science·Irene HanningSteven C Ricke
Mar 26, 2014·Vector Borne and Zoonotic Diseases·Antonio GargiuloLudovico Dipineto
Nov 2, 2018·Japanese Journal of Infectious Diseases·S M Lutful KabirShinji Yamasaki
Jan 11, 2019·Zoonoses and Public Health·Eugenia RosslerLaureano S Frizzo
Nov 25, 2006·Critical Reviews in Microbiology·James L Smith, Darrell O Bayles
Dec 3, 2008·Journal of Food Protection·Jessica L ThorsnessCatherine M Logue
Nov 26, 2010·Tropical Animal Health and Production·Arzu FindikAlper Ciftci
Apr 20, 2011·Avian Diseases·Ludovico DipinetoAlessandro Fioretti
Jun 2, 2014·Apoptosis : an International Journal on Programmed Cell Death·B CanonicoW Baffone
Apr 1, 2015·Foodborne Pathogens and Disease·Leonard KoolmanDeclan Bolton
Dec 21, 2016·Frontiers in Cellular and Infection Microbiology·Monika D ScuronBruce J Shenker
Mar 13, 2018·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Samantha J LawtonWalter M Boyce
Jul 28, 2018·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Herlice do Nascimento VerasAldo A M Lima
Aug 15, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Érica Chaves LúcioJosé Wilton Pinheiro
Jan 6, 2006·Molecular Diagnosis : a Journal Devoted to the Understanding of Human Disease Through the Clinical Application of Molecular Biology·Vasilios KordinasStylianos Chatzipanagiotou
Jul 24, 2004·Journal of Food Protection·Roger B HarveyDavid J Nisbet
Oct 27, 2012·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Eunju ShinYeonhee Lee
Jan 1, 2014·Applied and Environmental Microbiology·Allison M WeisAndrea K Townsend
Jun 4, 2015·Virulence·Anja KlančnikDarinka Vučković

❮ Previous
Next ❯

Related Concepts

Related Feeds

Campylobacteriosis (ASM)

Campylobacteriosis is caused by the bacteria Campylobacter jejuni and is a common cause of gastroenteritis in humans. Discover the latest research on Campylobacteriosis here.