PCR detection of Toxoplasma gondii DNA in CSF for the differential diagnosis of AIDS-related focal brain lesions

Journal of Medical Microbiology
A CingolaniA Antinori

Abstract

To evaluate whether the detection of Toxoplasma gondii DNA in CSF could contribute to the differential diagnosis of AIDS-related focal brain lesions, CSF samples from 88 HIV-infected patients (56 with focal brain lesions and 32 without) were tested prospectively by a nested PCR for the B1 gene of T. gondii. The assay had a detection limit of 10 trophozoite equivalents. Six of 18 patients with toxoplasmic encephalitis, but none of the 70 patients with other disorders, were PCR-positive (33.3% sensitivity and 100% specificity). Considering only those patients with cerebral toxoplasmosis from whom CSF was collected before or during the first week of antitoxoplasmic therapy, sensitivity rose to 50%. This was higher than the sensitivity in patients whose CSF was collected after the first week of treatment (odds ratio (OR) of 7.0; 95% CI: 0.46-218.2). The administration of antitoxoplasmic prophylaxis did not affect the PCR results. Patients with a poor response to therapy had a higher probability of detectable T. gondii DNA in their CSF (OR of 5.0; 95% CI: 0.37-86.6). All patients with other central nervous system disorders were PCR-negative. Despite the moderate sensitivity, the high specificity and positive predictive value (100%) ...Continue Reading

Citations

Nov 22, 2008·Der Nervenarzt·G Arendt, T Nolting
May 2, 2003·Nihon rinsho. Japanese journal of clinical medicine·Takuya Maeda
Jun 12, 2002·Transactions of the Royal Society of Tropical Medicine and Hygiene·Patrick Bastien
Oct 12, 2010·Clinical Microbiology Reviews·J L N BarrattD Stark
Apr 12, 2012·Clinical Microbiology Reviews·Florence Robert-Gangneux, Marie-Laure Dardé
Oct 9, 2004·Journal of Clinical Microbiology·José E VidalVera Lucia Pereira-Chioccola
Mar 10, 2009·Cerebrospinal Fluid Research·Yenisey AlfonsoDora Ginorio
Dec 10, 2009·Future Microbiology·Vera Lucia Pereira-ChioccolaChunlei Su
Mar 29, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Omar K SiddiqiIgor J Koralnik
Mar 24, 2009·Médecine et maladies infectieuses·Y PittnerD Peyramond
Jun 20, 2012·Lancet Neurology·Ik Lin TanJustin C McArthur
Feb 13, 2010·Journal of Medical Microbiology·Rafael T MesquitaVera L Pereira-Chioccola
Oct 22, 2010·The Brazilian Journal of Infectious Diseases : an Official Publication of the Brazilian Society of Infectious Diseases·Rafael Tonini MesquitaVera Lucia Pereira-Chioccola
Dec 12, 2012·Diagnostic Microbiology and Infectious Disease·Kei MikitaAkihiko Kawana
Aug 2, 2016·IDCases·J B CrenN Crochette
Sep 2, 2016·IDCases·Rita MaganoJosé Gabriel Saraiva da Cunha
Dec 10, 2014·Clinical Lymphoma, Myeloma & Leukemia·Adan Rios
May 6, 1998·Seminars in Ultrasound, CT, and MR·F L ChiangC M Mehringer
Oct 9, 2014·Topics in Magnetic Resonance Imaging : TMRI·Ravi V GottumukkalaRafael S Glikstein
Jul 30, 2019·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Yunyun LiJianjun Wang
May 19, 2016·Current Opinion in Infectious Diseases·Florence Robert-Gangneux, Sorya Belaz
Jan 11, 2003·Current Treatment Options in Neurology·Avindra Nath, Anthony P. Sinai
Jul 30, 2010·British Journal of Nursing : BJN·Tom Wingfield, Ed Wilkins
May 19, 2017·Brain Tumor Research and Treatment·Sang-Bok Lee, Tae-Gyu Lee
Aug 21, 2019·Journal of the International Association of Providers of AIDS Care·José Ernesto Vidal
Aug 23, 2001·Scandinavian Journal of Infectious Diseases·I JulanderB Evengård
Mar 17, 2020·Journal of Parasitic Diseases : Official Organ of the Indian Society for Parasitology·Farah Bokharaei-SalimBorna Salemi
Apr 15, 2021·Transactions of the Royal Society of Tropical Medicine and Hygiene·Khadijeh KhanalihaBorna Salemi
Jun 12, 2021·Expert Review of Anti-infective Therapy·Marie Gladys RobertHervé Pelloux

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.