PCR-DGGE assessment of the bacterial diversity of breast milk in women with lactational infectious mastitis.

BMC Infectious Diseases
Susana DelgadoJuan M Rodríguez

Abstract

Infectious mastitis is a common condition during lactation and in fact, represents one of the main causes leading to a precocious weaning. The number of studies dealing with lactational mastitis is low and, up to now, the etiological diagnosis is frequently made on the basis of unspecific clinical signs. The aim of this study was to investigate the microbial diversity of breast milk in 20 women with lactational mastitis employing culture-dependent and culture-independent (PCR-DGGE) approaches. Breast milk samples were cultured in different media to investigate the presence of bacteria and/or yeasts, and a total of 149 representative isolates were identified to the species level by 16S rRNA gene PCR sequencing. The microorganisms recovered were compared with those found by PCR-DGGE analysis. To identify the DGGE profiles two reference markers of different microbial species were constructed. Sequence analysis of unknown bands was also performed. Staphylococci were the dominant bacterial group and Staphylococcus epidermidis was the dominant species. In a lower number of samples, other bacteria (mainly streptococci and a few gram-negative species) were also identified. Globally, PCR-DGGE results showed a good correlation with those...Continue Reading

References

Jan 1, 1985·Acta Obstetricia Et Gynecologica Scandinavica·A C ThomsenF Løve Jepsen
Feb 26, 2000·Infection and Immunity·S Zhang, C W Maddox
Jan 13, 2001·Journal of Human Lactation : Official Journal of International Lactation Consultant Association·K L Osterman, V A Rahm
Jan 16, 2002·American Journal of Epidemiology·Betsy FoxmanKendra Schwartz
Apr 5, 2002·Microbes and Infection·Cuong Vuong, Michael Otto
Aug 13, 2003·Journal of Applied Microbiology·M P Heikkilä, P E J Saris
Aug 26, 2003·Archives of Disease in Childhood·C MichieW Lynn
Dec 6, 2003·The Journal of Pediatrics·Rocío MartínJuan M Rodríguez
Feb 18, 2004·The Breast : Official Journal of the European Society of Mastology·A R Carmichael, J M Dixon
Aug 6, 2004·Applied and Environmental Microbiology·Shea S Beasley, Per E J Saris
Aug 7, 2004·Journal of Human Lactation : Official Journal of International Lactation Consultant Association·Jimi Francis-MorrillKathryn G Dewey
Feb 1, 2005·Journal of Human Lactation : Official Journal of International Lactation Consultant Association·Rocío MartínJuan M Rodríguez
Mar 2, 2005·Veterinary Microbiology·Janaína dos Santos NascimentoMaria do Carmo de Freire Bastos
Mar 15, 2006·Veterinary Microbiology·B-M ThorbergMarie-Louise Danielsson-Tham
Jul 11, 2006·International Journal of Antimicrobial Agents·Wilma ZiebuhrSvetlana Kozitskaya
Jan 17, 2007·Research in Microbiology·Rocío MartínJuan M Rodríguez
Mar 3, 2007·Pediatrics·Pablo F PerezAnne Donnet-Hughes

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Citations

Sep 29, 2011·Journal of Mammary Gland Biology and Neoplasia·G Andres Contreras, Juan Miguel Rodríguez
Jan 1, 2009·Irish Veterinary Journal·Rn Zadoks, Jl Fitzpatrick
Jun 25, 2013·Breastfeeding Medicine : the Official Journal of the Academy of Breastfeeding Medicine·Ann WittSteven Zyzanski
Jun 1, 2014·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Rosa Vázquez-FresnoCristina Andres-Lacueva
Aug 28, 2014·The British Journal of Nutrition·Henrike BergmannHania Szajewska
Jul 5, 2012·Women's Health·Camilla UrbaniakGregor Reid
Nov 28, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Leónides FernándezJuan Miguel Rodríguez
Oct 30, 2015·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Olga Pustotina
Dec 19, 2015·Food Microbiology·Helena Lucena-Padrós, José Luis Ruiz-Barba
Aug 1, 2015·Journal of Mammary Gland Biology and Neoplasia·Felicia GoldsmithSamara L Freeman
Feb 21, 2009·Letters in Applied Microbiology·M C ColladoJ M Rodríguez
Jun 1, 2010·International Journal of Systematic and Evolutionary Microbiology·Virginia MartínAntonio Maldonado-Barragán
Jan 28, 2014·Beneficial Microbes·L FernándezJ M Rodríguez
Apr 12, 2011·FEMS Immunology and Medical Microbiology·Susana DelgadoJuan M Rodríguez
May 26, 2016·Diagnostic Microbiology and Infectious Disease·Daniel TenaJuan Antonio Sáez-Nieto
May 15, 2016·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·W MutschlechnerC Lass-Flörl
May 8, 2015·Journal of Human Lactation : Official Journal of International Lactation Consultant Association·Esther JiménezJuan M Rodríguez
Aug 12, 2015·Journal of Human Lactation : Official Journal of International Lactation Consultant Association·Virginia MartínMaría Marín
Jul 14, 2017·PloS One·Esther JiménezJuan M Rodríguez
Jan 20, 2018·Medical Microbiology and Immunology·Angeliki AngelopoulouR Paul Ross
Apr 27, 2019·Future Microbiology·Amadou TogoMatthieu Million
Aug 15, 2012·Nature Reviews. Gastroenterology & Hepatology·Samuli RautavaErika Isolauri
Aug 12, 2017·Scientific Reports·Shriram H PatelAnju P Kunjadiya
Nov 7, 2019·JBI Database of Systematic Reviews and Implementation Reports·Melissa BarkerMary Steen
Apr 14, 2016·Maternal and Child Health Journal·Leónides FernándezMaría Marín
Jul 12, 2019·Frontiers in Microbiology·Lorena RuizJuan Miguel Rodriguez
Aug 20, 2020·JBI Evidence Synthesis·Melissa BarkerMary Steen
Apr 19, 2017·Journal of Human Lactation : Official Journal of International Lactation Consultant Association·Pilar MedianoMaría Marín
Aug 22, 2017·Frontiers in Microbiology·Nicole T CachoGraciela L Lorca
Nov 8, 2018·Nutrients·Anna Ojo-OkunolaElloise du Toit
Dec 18, 2020·Frontiers in Cellular and Infection Microbiology·Leónides FernándezJuan M Rodríguez

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Datasets Mentioned

BETA
AM779062
AM779086
AM774573
AM774577
AM774579
AM774586

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

BLAST

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